Nastruzzi C, Barbieri R, Ferroni R, Guarneri M, Spandidos D, Gambari R
Dip. di Scienze Farmaceutiche, Universita di Ferrara, Italy.
Cancer Lett. 1990 Apr 20;50(2):93-102. doi: 10.1016/0304-3835(90)90237-r.
In the present paper we have investigated the effects of aromatic tetra-amidines on attachment, oriented migration and in vitro invasiveness of the Chinese Hamster FH06T1-1 fibroblast lung cell line, transformed with the activated human T24-Ha-ras-1 oncogene. The FH06T1-1 cell line is tumorigenic in nude mice and displays growth properties and biological features clearly distinct from those of the FH06N1-1 cell line, obtained after transfection of the same fibroblast cells with the normal Ha-ras-1 proto-oncogene. Attachment, oriented migration and invasiveness were analysed by culturing the cells on a reconstituted extracellular matrix, composed of collagen IV, laminin, entactin and heparan sulphate proteoglycans. The results obtained demonstrate that oriented migration is performed only by FH06T1-1 cells and that tetra-benzamidines are effective inhibitors of oriented migration and "in vitro" invasiveness of these tumorigenic cells. These findings should encourage further studies on the possible antimetastatic effects of these antiproteinase tetra-benzamidines on experimental animals.
在本论文中，我们研究了芳香族四脒对中国仓鼠FH06T1-1成纤维细胞肺癌细胞系的黏附、定向迁移和体外侵袭的影响，该细胞系由激活的人T24-Ha-ras-1癌基因转化而来。FH06T1-1细胞系在裸鼠中具有致瘤性，并且表现出与FH06N1-1细胞系明显不同的生长特性和生物学特征，后者是用正常的Ha-ras-1原癌基因转染相同的成纤维细胞后获得的。通过将细胞培养在由IV型胶原、层粘连蛋白、巢蛋白和硫酸乙酰肝素蛋白聚糖组成的重组细胞外基质上来分析黏附、定向迁移和侵袭。所获得的结果表明，只有FH06T1-1细胞能够进行定向迁移，并且四苯甲脒是这些致瘤细胞定向迁移和“体外”侵袭的有效抑制剂。这些发现应该会促使人们进一步研究这些抗蛋白酶四苯甲脒对实验动物可能的抗转移作用。
