Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, CCT La Plata-CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):471-6. doi: 10.1016/j.colsurfb.2011.07.035. Epub 2011 Jul 23.
Mg-based materials are promising for orthopedic, dental, and cardiovascular applications but their high degradation rate in vivo (release of Mg ions and debris particles) is cause of great concern. Protective treatments involving fluoride conversion coatings have been proposed in order to reduce corrosion rates. The aim of this study was to evaluate Mg debris biodegradation and its possible cytotoxic effects on osteoblastic cells in situ. Neutral Red dying and Acridine Orange staining techniques were used as endpoints to analyse the cytotoxic effects at 100-2000 μg/mL concentration range. Results showed a marked variation of Mg ion concentration in the culture medium after different exposure periods (1, 2, or 24h). Interestingly, the release rate of magnesium ions was dependent on the presence or absence fluoride treatment. Adverse effects induced by ≥1000 μg/mL MP doses and Mg ion concentrations higher than 480 μg/mL were observed on cells. Results showed significant differences between the concentration of Mg ions in the presence and absence of cells. This fact reveals a dynamic equilibrium mediated by Mg ion input and output in the cells that leads to the change in MP corrosion rates. Fluoride release from conversion coatings did not show cytotoxic effects.
镁基材料在骨科、牙科和心血管应用方面具有广阔的前景，但它们在体内的高降解率（镁离子和碎片颗粒的释放）引起了极大的关注。为了降低腐蚀速率，人们提出了涉及氟化物转化涂层的保护处理方法。本研究旨在评估镁碎片的生物降解及其对原位成骨细胞的潜在细胞毒性作用。中性红染色和吖啶橙染色技术被用作分析 100-2000μg/ml 浓度范围内细胞毒性作用的终点。结果表明，在不同的暴露时间（1、2 或 24h）后，培养基中镁离子的浓度有明显的变化。有趣的是，镁离子的释放速率取决于氟化物处理的存在与否。在≥1000μg/ml MP 剂量和镁离子浓度高于 480μg/ml 的情况下，观察到细胞受到不良影响。结果表明，在有细胞和无细胞的情况下，镁离子浓度存在显著差异。这一事实揭示了由细胞内镁离子输入和输出介导的动态平衡，导致 MP 腐蚀速率的变化。转化涂层中氟化物的释放没有显示出细胞毒性作用。
