Roth Isabelle, Schumacher Stephan, Basler Tina, Baumert Kathrin, Seitz Jan-Marten, Evertz Florian, Müller Peter Paul, Bäumer Wolfgang, Kietzmann Manfred
Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559, Hannover, Germany.
Elanco Animal Health, Lilly Deutschland GmbH, Werner-Reimers-Str. 2-4, 61352, Bad Homburg, Germany.
Prog Biomater. 2015 Mar;4(1):21-30. doi: 10.1007/s40204-014-0032-9. Epub 2014 Dec 6.
Magnesium is currently under investigation as a prospective biodegradable implant material. Biodegradation of magnesium causes a release of magnesium, hydroxide ions and hydrogen gas but it can also lead to the formation of particulate debris. Implant-derived particles may have immunotoxic effects. To investigate the influence of magnesium-derived particles on the immune functions of primary macrophages, up to 500 μg/ml magnesium or magnesium corrosion particles were added to the cell culture medium. No major effects were observed on cell viability and on the release of the proinflammatory cytokine tumor necrosis factor (TNF)α. In addition, the ability of macrophages to stimulate proliferation of allogenic lymphocytes in a mixed leukocyte reaction remained unaffected. When macrophages were incubated with magnesium particles and then infected with the apathogenic Mycobacterium smegmatis, infection-induced TNFα secretion from murine macrophages was inhibited but not from human macrophages. However, the bactericidal activity of either cell type was not influenced. In conclusion, magnesium-related particles did not restrict the immune function of macrophages, suggesting that magnesium implants and corrosion particles derived thereof are highly biocompatible and have a low inflammatory potential.
镁目前作为一种潜在的可生物降解植入材料正在研究中。镁的生物降解会导致镁、氢氧根离子和氢气的释放，但也可能导致颗粒碎片的形成。植入物衍生的颗粒可能具有免疫毒性作用。为了研究镁衍生颗粒对原代巨噬细胞免疫功能的影响，将高达500μg/ml的镁或镁腐蚀颗粒添加到细胞培养基中。未观察到对细胞活力和促炎细胞因子肿瘤坏死因子（TNF）α释放的主要影响。此外，巨噬细胞在混合白细胞反应中刺激同种异体淋巴细胞增殖的能力仍未受影响。当巨噬细胞与镁颗粒孵育然后感染无致病性耻垢分枝杆菌时，感染诱导的小鼠巨噬细胞TNFα分泌受到抑制，但人巨噬细胞不受影响。然而，两种细胞类型的杀菌活性均未受影响。总之，镁相关颗粒并未限制巨噬细胞的免疫功能，这表明镁植入物及其衍生的腐蚀颗粒具有高度生物相容性且炎症潜力低。
