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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Harder G, Jamieson J C, Woloski B M

Department of Chemistry, University of Manitoba, Winnipeg, Canada.

Int J Biochem. 1990;22(1):11-4. doi: 10.1016/0020-711x(90)90070-j.

Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase was assayed in FAZA hepatoma cells and the cell culture medium following growth of cells in presence of dexamethasone and phorbol ester. 2. There was about a seven-fold increase in sialyltransferase activities in cells and medium in presence of dexamethasone with the maximum effect occurring at 10(-6)-10(-7) M dexamethasone. 3. The presence of 10(-6) M phorbol ester in the culture medium increased sialyltransferase activities in cells and medium by ca 40% over the values found with dexamethasone alone. 4. The use of the FAZA hepatoma cell line for studies on sialyltransferase is compared with the primary hepatocyte system reported on earlier (Woloski et al., 1986).

在存在地塞米松和佛波酯的情况下培养FAZA肝癌细胞后，测定细胞和细胞培养基中的半乳糖β1-4N-乙酰葡糖胺α2-6唾液酸转移酶。2. 在地塞米松存在的情况下，细胞和培养基中的唾液酸转移酶活性增加了约7倍，在10(-6)-10(-7)M地塞米松时达到最大效应。3. 培养基中10(-6)M佛波酯的存在使细胞和培养基中的唾液酸转移酶活性比单独用地塞米松时的值增加约40%。4. 将FAZA肝癌细胞系用于唾液酸转移酶研究与早期报道的原代肝细胞系统（Woloski等人，1986年）进行了比较。

Harder G, Jamieson J C, Woloski B M

Department of Chemistry, University of Manitoba, Winnipeg, Canada.

Int J Biochem. 1990;22(1):11-4. doi: 10.1016/0020-711x(90)90070-j.

Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase was assayed in FAZA hepatoma cells and the cell culture medium following growth of cells in presence of dexamethasone and phorbol ester. 2. There was about a seven-fold increase in sialyltransferase activities in cells and medium in presence of dexamethasone with the maximum effect occurring at 10(-6)-10(-7) M dexamethasone. 3. The presence of 10(-6) M phorbol ester in the culture medium increased sialyltransferase activities in cells and medium by ca 40% over the values found with dexamethasone alone. 4. The use of the FAZA hepatoma cell line for studies on sialyltransferase is compared with the primary hepatocyte system reported on earlier (Woloski et al., 1986).

在存在地塞米松和佛波酯的情况下培养FAZA肝癌细胞后，测定细胞和细胞培养基中的半乳糖β1-4N-乙酰葡糖胺α2-6唾液酸转移酶。2. 在地塞米松存在的情况下，细胞和培养基中的唾液酸转移酶活性增加了约7倍，在10(-6)-10(-7)M地塞米松时达到最大效应。3. 培养基中10(-6)M佛波酯的存在使细胞和培养基中的唾液酸转移酶活性比单独用地塞米松时的值增加约40%。4. 将FAZA肝癌细胞系用于唾液酸转移酶研究与早期报道的原代肝细胞系统（Woloski等人，1986年）进行了比较。