Dept. of Pathology, University of Western Ontario, Schulich School of Medicine and Dentistry, London, Ontario, Canada.
Phytomedicine. 2011 Oct 15;18(13):1110-7. doi: 10.1016/j.phymed.2011.06.013. Epub 2011 Aug 16.
Ginseng (Araliaceae), demonstrates widespread biological effects because of its purported antioxidant and other properties. The present study was undertaken to investigate the effects of American ginseng root extract on glucose-induced oxidative stress and associated oxidative damage to human umbilical vein endothelial cells (HUVECs).
Following pretreatment with various concentrations of ginseng (alcoholic extract), HUVECs were incubated with various concentrations of d-glucose ranging from 5 to 25mmol/l for 24h. l-Glucose was used at a concentration of 25mmol/l as a control.
Glucose-induced oxidative stress detected by intracellular reactive oxygen species accumulation, superoxide anion generation and DNA damage in HUVECs were significantly prevented by ginseng. Treatment of HUVECs with ginseng further led to significant prevention of glucose-induced NF-κB activation. Glucose-induced increase in fibronectin (FN), EDB(+)FN (a splice variant of FN), endothelin-1 (ET-1) and vascular endothelial growth factor (VEGF) mRNAs and protein levels were also prevented by ginseng treatment.
These data indicate that American ginseng prevented glucose-induced damage in the HUVECs through its antioxidant properties.
人参（五加科）由于其具有抗氧化和其他特性而显示出广泛的生物学效应。本研究旨在研究人参根提取物对葡萄糖诱导的氧化应激及相关人脐静脉内皮细胞（HUVEC）氧化损伤的影响。
用人参（酒精提取物）的不同浓度进行预处理后，将 HUVEC 与 5 至 25mmol/l 范围的不同浓度的 d-葡萄糖孵育 24 小时。l-葡萄糖的浓度为 25mmol/l，作为对照。
人参可显著预防葡萄糖诱导的 HUVEC 内活性氧物种积累、超氧阴离子生成和 DNA 损伤引起的氧化应激。人参处理 HUVEC 还可显著预防葡萄糖诱导的 NF-κB 激活。葡萄糖诱导的纤维连接蛋白（FN）、EDB(+)FN（FN 的剪接变体）、内皮素-1（ET-1）和血管内皮生长因子（VEGF）mRNA 和蛋白水平的增加也被人参处理所阻止。
这些数据表明，人参通过其抗氧化特性预防了葡萄糖诱导的 HUVEC 损伤。
