Naval M V, Gómez-Serranillos M P, Carretero M E, Villar A M
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
J Ethnopharmacol. 2007 Jun 13;112(2):262-70. doi: 10.1016/j.jep.2007.03.010. Epub 2007 Mar 12.
A standardized aqueous extract of Panax ginseng radix was tested for its antioxidant effect on primary astrocytes culture on an oxidant stress model generated by H(2)O(2). The results indicated that this extract had a significant effect on the reduction of astrocytic death induced by H(2)O(2). Dose-response experiments revealed that this ginseng extract increased cell viability at a wide range of concentrations. Therefore, we investigated the effects of this extract on antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidases (GPx) and glutathione reductase (GR), on glutathione content (reduced and oxidized forms and red/ox index) and on the intracellular reactive oxygen species (ROS) formation. Exposure of astrocytes to H(2)O(2) decreased the activities of antioxidant enzymes, and increased ROS formation. Ginseng root extract reversed the effect of almost all of these parameters in H(2)O(2)-injured primary cultures of rat astrocytes.
在由过氧化氢（H₂O₂）产生的氧化应激模型上，对标准化人参根水提取物对原代星形胶质细胞培养物的抗氧化作用进行了测试。结果表明，该提取物对减少H₂O₂诱导的星形胶质细胞死亡具有显著作用。剂量反应实验表明，这种人参提取物在很宽的浓度范围内都能提高细胞活力。因此，我们研究了该提取物对过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）等抗氧化酶、谷胱甘肽含量（还原型和氧化型以及还原/氧化指数）以及细胞内活性氧（ROS）形成的影响。将星形胶质细胞暴露于H₂O₂会降低抗氧化酶的活性，并增加ROS的形成。人参根提取物逆转了几乎所有这些参数在H₂O₂损伤的大鼠星形胶质细胞原代培养物中的作用。
