Mizoguchi I, Nakamura M, Takahashi I, Kagayama M, Mitani H
Department of Orthodontics, School of Dentistry, Tohoku University, Sendai, Japan.
Histochemistry. 1990;93(6):593-9. doi: 10.1007/BF00272201.
Immunohistochemical localization of type I and type II collagens was examined in the rat mandibular condylar cartilage (as the secondary cartilage) and compared with that in the tibial growth plate (as the primary cartilage) using plastic embedded tissues. In the condylar cartilage, type I collagen was present not only in the extracellular matrix (ECM) of the fibrous, proliferative, and transitional cell layers, but also in the ECM of the maturative and hypertrophic cell layers. Type II collagen was present in the ECM of the maturative and hypertrophic cell layers. In the growth plate, type II collagen was present in the ECM of whole cartilaginous layers; type I collagen was not present in the cartilage but in the perichondrium and the bone matrices. These results indicate that differences exist in the components of the ECM between the primary and secondary cartilages. It is suggested that these two tissues differ in the developmental processes and/or in the reactions to their own local functional needs.
利用塑料包埋组织，检测了大鼠下颌髁突软骨（作为次级软骨）中I型和II型胶原蛋白的免疫组化定位，并与胫骨生长板（作为初级软骨）中的进行了比较。在髁突软骨中，I型胶原蛋白不仅存在于纤维层、增殖层和过渡细胞层的细胞外基质（ECM）中，也存在于成熟层和肥大细胞层的ECM中。II型胶原蛋白存在于成熟层和肥大细胞层的ECM中。在生长板中，II型胶原蛋白存在于整个软骨层的ECM中；I型胶原蛋白不存在于软骨中，而是存在于软骨膜和骨基质中。这些结果表明，初级软骨和次级软骨的ECM成分存在差异。提示这两种组织在发育过程和/或对自身局部功能需求的反应方面存在差异。
