G Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad 500 028, AP, India.
J Ethnopharmacol. 2011 Oct 11;137(3):1353-9. doi: 10.1016/j.jep.2011.07.067. Epub 2011 Aug 6.
The bark of Tecomella undulata is primarily used in the treatment of syphilis, painful swellings and cancer by traditional healers. Also, it is claimed to be useful in treating urinary discharges, enlargement of spleen, leucorrhoea, leukoderma, tumors, liver disorders, gonorrhea, gout and promotes wound healing in Indian traditional system of medicine.
To establish a scientific validation for the antitumor effects of Tecomella undulata bark and explore the mechanistic pathway in chronic myeloid leukemia cell line, K562. The study was further extended to standardize the extract using quercetin as biomarker.
Induction of apoptosis by chloroform extract of Tecomella undulata bark (CTUB) was determined by MTT, Annexin V and caspase activation assays. The cell cycle analysis was done by flow cytometer and nuclear staining by DAPI. The standardization of the extract was performed through reverse phase-HPLC method under PDA detection.
Results clearly showed the induction of apoptosis by CTUB in K562 cells. The effect was found to be dose dependent, having IC(50) of 30 μg/ml with activation of FAS, FADD, caspase 8, caspase 3/7 and fragmentation of DNA. The bioactive CTUB was determined to possess 0.03% (w/w) of quercetin.
The investigation clearly demonstrated the potential antitumor effect of CTUB, thereby validating the traditional claim. Quercetin, known to have anticancer activity is being reported and quantified for the first time from the bark of Tecomella undulata.
传统行医者主要用紫薇 Tecomella undulata 的树皮来治疗梅毒、肿痛和癌症。此外，据说它对治疗尿漏、脾肿大、白带、白癜风、肿瘤、肝脏疾病、淋病、痛风和促进伤口愈合也有用，这在印度传统医学体系中得到了应用。
为紫薇 Tecomella undulata 树皮的抗肿瘤作用建立科学验证，并探讨其在慢性髓性白血病细胞系 K562 中的作用机制。该研究还进一步扩展到使用槲皮素作为生物标志物来对提取物进行标准化。
通过 MTT、Annexin V 和 caspase 激活测定来确定氯仿提取物 CTUB 对 Tecomella undulata 树皮诱导的细胞凋亡。通过流式细胞仪进行细胞周期分析，并通过 DAPI 核染色进行分析。通过 PDA 检测下的反相高效液相色谱法对提取物进行标准化。
结果清楚地表明 CTUB 在 K562 细胞中诱导了细胞凋亡。该作用呈剂量依赖性，IC50 为 30 μg/ml，同时激活了 Fas、FADD、caspase 8、caspase 3/7，并导致 DNA 片段化。生物活性 CTUB 被确定含有 0.03%（w/w）的槲皮素。
研究清楚地表明 CTUB 具有潜在的抗肿瘤作用，从而验证了传统说法。槲皮素具有抗癌活性，这是首次从紫薇 Tecomella undulata 的树皮中得到报道和定量。
