Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan 430030, China.
Environ Toxicol Pharmacol. 2011 Sep;32(2):122-7. doi: 10.1016/j.etap.2011.04.003. Epub 2011 Apr 17.
This study was to appraise the anti-inflammatory and free radical scavenging activities of ethanol extracts of Ilex ficoidea Hemsl (EIFH) and Ilex centrochinensis S.Y. Hu (EICC). Anti-inflammatory activities were evaluated using xylene-induced ear edema and the cotton pellet granuloma tests. Some antioxidants produced during chronic inflammation were quantitated and the DPPH, superoxide anion and hydroxyl free radicals scavenging activities in vitro were also investigated. EICC showed a significant anti-inflammatory effect during the acute inflammation and chronic inflammation without any acute oral toxicity. However, EIFH exhibited a negligible anti-acute inflammatory and a moderate anti-chronic inflammatory activity. EICC significantly inhibited the formation of MDA and markedly raised the activities of SOD, CAT and GSH-Px. EIFH could reduce the level of MDA and elevate the activity of SOD only at the high dose. The free radical scavenging activities of EICC are higher than that of EIFH. These results suggest that EICC possesses significant anti-inflammatory and free radical scavenging activities. The anti-inflammatory mechanism is attributed, in part, to enhance the activities of antioxidant enzymes.
本研究旨在评价榕叶冬青（EIFH）和毛冬青（EICC）乙醇提取物的抗炎和自由基清除活性。采用二甲苯致耳肿胀和棉垫肉芽肿试验评价抗炎活性。定量测定慢性炎症过程中产生的一些抗氧化剂，并研究其在体外对 DPPH、超氧阴离子和羟自由基的清除活性。EICC 在急性和慢性炎症中均表现出显著的抗炎作用，且无急性口服毒性。然而，EIFH 对急性炎症的抑制作用可忽略不计，对慢性炎症的抑制作用中等。EICC 显著抑制 MDA 的形成，显著提高 SOD、CAT 和 GSH-Px 的活性。EIFH 仅在高剂量时才能降低 MDA 水平并提高 SOD 活性。EICC 的自由基清除活性高于 EIFH。这些结果表明，EICC 具有显著的抗炎和自由基清除活性。抗炎机制部分归因于增强抗氧化酶的活性。
