College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Bioresour Technol. 2011 Oct;102(19):9026-32. doi: 10.1016/j.biortech.2011.07.076. Epub 2011 Jul 27.
The aim of this study was to compare the relative contribution of ammonia-oxidizing archaea (AOA) and bacteria (AOB) to nitrification during agricultural waste composting. The AOA and AOB amoA gene abundance and composition were determined by quantitative PCR and denaturing gradient gel electrophoresis (DGGE), respectively. The results showed that the archaeal amoA gene was abundant throughout the composting process, while the bacterial amoA gene abundance decreased to undetectable level during the thermophilic and cooling stages. DGGE showed more diverse archaeal amoA gene composition when the potential ammonia oxidation (PAO) rate reached peak values. A significant positive relationship was observed between the PAO rate and the archaeal amoA gene abundance (R²=0.554; P<0.001), indicating that archaea dominated ammonia oxidation during the thermophilic and cooling stages. Bacteria were also related to ammonia oxidation activity (R²=0.503; P=0.03) especially during the mesophilic and maturation stages.
本研究旨在比较氨氧化古菌（AOA）和细菌（AOB）在农业废弃物堆肥过程中对硝化作用的相对贡献。通过定量 PCR 和变性梯度凝胶电泳（DGGE）分别测定 AOA 和 AOB amoA 基因丰度和组成。结果表明，在整个堆肥过程中，古菌 amoA 基因丰富，而细菌 amoA 基因丰度在高温和冷却阶段下降到无法检测的水平。当潜在氨氧化（PAO）速率达到峰值时，DGGE 显示出更多样化的古菌 amoA 基因组成。PAO 速率与古菌 amoA 基因丰度之间存在显著正相关关系（R²=0.554；P<0.001），表明在高温和冷却阶段，古菌主导了氨氧化作用。细菌也与氨氧化活性有关（R²=0.503；P=0.03），特别是在中温阶段和成熟阶段。
