Department of Biochemistry, Sri Krishnadevaraya University, Anantapur-515 003, Andhra Pradesh, India.
Indian J Pharmacol. 2011 Jul;43(4):414-8. doi: 10.4103/0253-7613.83112.
In the present study, we have evaluated the antihyperglycemic, hypolipidemic and antioxidant activities of aqueous extract of Phyllanthus amarus (PAAEt) in streptozotocin (STZ)-induced diabetic rats.
PAAEt was administered at 200 mg/kg body weight/day to normal treated (NT-group) and STZ-induced diabetic treated rats (DT-group) by gavage for eight weeks. During the experimental period, blood was collected from fasted rats at 10 days intervals and plasma glucose level was estimated. The plasma lipid profile was estimated at the end of experimental period. After the treatment, period kidney lipid peroxidation (LPO), protein oxidation and reduced glutathione (GSH) were estimated and antioxidant enzymes viz., glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) were also assayed.
The significant decrease in the body weight, hyperglycemia and hyperlipidemia observed in STZ-induced diabetic rats (D-group) were rectified with PAAEt treatment in diabetic treated group (DT-group). D-group rats showed increased renal oxidative stress with increased LPO and protein oxidation. DT-group showed a significant decrease in renal LPO, protein oxidation and a significant increase in GSH content and GR, GPx and GST activities when compared with D-group. The activities of SOD and CAT decreased significantly in D-group, but were normalized in DT-group. Normal rats treated with PAAEt (NT-rats) showed a significant decrease in lipid profile, renal LPO and protein oxidation, with significant increase in renal GSH and activities of antioxidant enzymes compared to normal rats (N-group).
Our results demonstrated that PAAEt with its antidiabetic, hypolipidemic and antioxidant properties could be a potential herbal medicine in treating diabetes and renal problems.
本研究评估了叶下珠（PAAEt）水提物在链脲佐菌素（STZ）诱导的糖尿病大鼠中的降血糖、降血脂和抗氧化活性。
通过灌胃，PAAEt 以 200mg/kg 体重/天的剂量施用于正常治疗（NT 组）和 STZ 诱导的糖尿病治疗（DT 组）大鼠，共 8 周。在实验期间，每隔 10 天从禁食大鼠中采集血液并估计血浆葡萄糖水平。在实验期末估计血浆脂质谱。治疗后，估计肾脏脂质过氧化（LPO）、蛋白质氧化和还原型谷胱甘肽（GSH），并测定抗氧化酶，如谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）。
与 PAAEt 治疗的糖尿病治疗组（DT 组）相比，STZ 诱导的糖尿病大鼠（D 组）体重减轻、高血糖和高血脂显著改善。D 组大鼠肾脏氧化应激增加，LPO 和蛋白质氧化增加。与 D 组相比，DT 组肾脏 LPO、蛋白质氧化显著降低，GSH 含量和 GR、GPx 和 GST 活性显著增加。D 组 SOD 和 CAT 活性显著降低，但在 DT 组恢复正常。与正常大鼠（N 组）相比，用 PAAEt 治疗的正常大鼠（NT 大鼠）的脂质谱、肾脏 LPO 和蛋白质氧化显著降低，肾脏 GSH 和抗氧化酶活性显著增加。
我们的结果表明，具有抗糖尿病、降血脂和抗氧化特性的 PAAEt 可能是治疗糖尿病和肾脏问题的潜在草药。
