双氧水（H2O2）在白化大鼠中的长时间暴露及尼泊尔鬼臼对氧化应激的影响。

Long dose exposure of hydrogen peroxide (H2O2) in albino rats and effect of Podophyllum hexandrum on oxidative stress.

机构信息

Department of Biochemistry, University of Kashmir, Srinagar, India.

出版信息

Eur Rev Med Pharmacol Sci. 2011 Aug;15(8):906-15.

Abstract

OBJECTIVES

The effect of Podophyllum hexandrum methanolic extract and alpha-tocopherol in reducing oxidative stress in male albino rats was evaluated.

MATERIALS AND METHODS

Lipid peroxidation was monitored by measuring malondialdehyde (MDA) level in different tissues of rats. Activities of free radical scavenging enzymes (superoxide dismutase, glutathione peroxidase and catalase) were determined using H2O2 decomposition.

RESULTS

Results showed that administration of H2O2 (0.1%) in drinking water of the rats, for 25 weeks, increased the malondialdehyde levels in liver, kidney and lung tissues of all the rats. However, rats receiving Podophyllum hexandrum extract and alpha-tocopherol had lower MDA levels in a dose dependent manner, which indicates decreased lipid peroxidation in these rats. Increase in the catalase activity appears to be a response to H2O2 accumulation. The decrease in the activity of catalase and increase in the activity of superoxide dismutase and glutathione peroxidase in different organs of the rats receiving Podophyllum hexandrum extract and alpha-tocopherol indicates the protective effect of the plant in combating oxidative stress undergone by the rats.

OBJECTIVES

Rhizome of Podophyllum hexandrum have been ethnomedically claimed to possess a wide array of biological activities including anticancer activity.

MATERIALS AND METHODS

To verify the folklore claim, this study was performed in a six Human carcinoma cell lines, Lung (A-549), Prostate (PC-3), Colon (Colo-25), Breast (MCF-7), Neuroblastoma (IMR-32) and CNS (SF-295) MCF-7 and MDA-MB-231.

RESULTS

Methanol and 70% ethanolic extracts of the rhizome of Podophyllum hexandrum showed highest cytotoxic effect on MCF-7 (Breast) and Colo-25 (Colon) cell line, as determined with sulforhodamine-B (SRB) assay.

CONCLUSIONS

These findings 1 - showed that Podophyllum hexandrum extract may ameliorate H2O2 induced oxidative stress by decreasing lipid peroxidation via alteration of the antioxidant defense system of the rats. 2 - these data also showed the anticancer activity of the plant extracts on different human cancer cell lines. However, further investigation is needed to assess the molecular mechanisms mediated anticancer activities of this plant.

摘要

目的

评估尼泊尔鬼臼甲醇提取物和 α-生育酚对减少雄性白化大鼠氧化应激的影响。

材料和方法

通过测量大鼠不同组织中丙二醛（MDA）的水平来监测脂质过氧化。使用 H2O2 分解来测定自由基清除酶（超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶）的活性。

结果

结果表明，在大鼠饮用水中添加 H2O2（0.1%），连续 25 周，增加了所有大鼠肝脏、肾脏和肺组织中的 MDA 水平。然而，接受尼泊尔鬼臼提取物和 α-生育酚的大鼠 MDA 水平呈剂量依赖性降低，这表明这些大鼠的脂质过氧化减少。过氧化氢酶活性的增加似乎是对 H2O2 积累的一种反应。接受尼泊尔鬼臼提取物和 α-生育酚的大鼠不同器官中过氧化氢酶活性的降低和超氧化物歧化酶和谷胱甘肽过氧化物酶活性的增加表明该植物具有对抗大鼠氧化应激的保护作用。