候选基因中的单核苷酸多态性与山羊的抗感染能力显著相关。
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to infection in goats.
作者信息
Alam Mahmuda Bilkis Bintee, Omar Abdullah Ibne, Faruque Md Omar, Notter David Russell, Periasamy Kathiravan, Mondal Md Motahar Hussain, Sarder Md Jalal Uddin, Shamsuddin Md, Cao Jianhua, Du Xiaoyong, Wu Zhenyang, Zhao Shuhong
机构信息
1Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.
2National Engineering Laboratory for Animal Breeding, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.
出版信息
J Anim Sci Biotechnol. 2019 Mar 15;10:30. doi: 10.1186/s40104-019-0327-8. eCollection 2019.
BACKGROUND
Haemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, , which is highly pathogenic in small ruminants The aim of this study was to identifying single-nucleotide polymorphisms (SNP) that were associated with fecal egg counts (FEC) and could be used as markers to identify resistance to in goats.
RESULTS
Ten novel variants in the , and genes were associated with FEC in goats with a nominal significance level of < 0.05. Two missense mutation in the exon region of the caprine gene resulted in replacement of arginine with cysteine at position 9473550 (R9473550C) and aspartic acid with glutamic acid at position 9473870 (D9473870E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content (), effective allele number (), and heterozygosity () were greatest for the _197_A > G SNP locus in all goat breeds. Pairwise coefficients of linkage disequilibrium (D´, ) revealed complete LD ( = 1) between significant SNP polymorphisms in and and strong LD ( = 0.93 and 0.98) between polymorphisms in and , respectively. Correlation coefficient () between FEC and body weight (BW) was significantly positive ( = 0.56***, < 0.001) but that between FEC and packed cell volume (PCV) was negatively significant ( = - 0.47**, < 0.01) in the total population of goats. On the other hand, correlation coefficient () between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within , and were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of mRNA was higher ( < 0.001) for resistant, compared to susceptible, groups of goats for all candidate genes except .
CONCLUSIONS
This study identified SNP markers that can potentially be used in marker-assisted selection programs to develop goat breeds that are resistant to
背景
血矛线虫病是潮湿的热带和亚热带地区山羊生产中的一个主要经济问题。该病由一种皱胃线虫引起，这种线虫对小型反刍动物具有高度致病性。本研究的目的是鉴定与粪便虫卵计数（FEC）相关的单核苷酸多态性（SNP），并将其用作鉴定山羊对血矛线虫抗性的标记。
结果
在、和基因中发现了10个新的变异与山羊的FEC相关，其名义显著性水平为<0.05。山羊基因外显子区域的两个错义突变导致9473550位的精氨酸被半胱氨酸取代（R9473550C），9473870位的天冬氨酸被谷氨酸取代（D9473870E）。在各自的基因型中，中国山羊品种的FEC显著高于孟加拉国山羊品种。所有山羊品种中，_197_A>G SNP位点的多态信息含量（）、有效等位基因数（）和杂合度（）最大。成对连锁不平衡系数（D´，）显示，和中的显著SNP多态性之间存在完全连锁不平衡（=1），和中的多态性之间分别存在强连锁不平衡（=0.93和0.98）。在山羊总群体中，FEC与体重（BW）之间的相关系数（）显著为正（=0.56***，<0.001），但FEC与红细胞压积（PCV）之间的相关系数为负（=-0.47**，<0.01）。另一方面，在山羊总群体中，BW与PCV之间的相关系数不显著。关联分析表明，、和中的单倍型与FEC显著相关。定量实时PCR显示，除基因外，所有候选基因抗性山羊组的mRNA相对表达均高于易感山羊组（<0.001）。
结论
本研究鉴定了SNP标记，这些标记有可能用于标记辅助选择计划，以培育抗血矛线虫的山羊品种。
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