Gans L A, Lee S F, Lemp M A, Pepose J S
Department of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri 63110.
Am J Ophthalmol. 1990 Apr 15;109(4):474-7. doi: 10.1016/s0002-9394(14)74616-1.
Freshly frozen conjunctival tissue from premenopausal and postmenopausal women and male subjects were processed for estrogen and progesterone receptors by using monoclonal antibodies and a peroxidase-antiperoxidase technique. No immunocytochemical staining was localized in the nuclei of the cells treated with the monoclonal antibodies to human estrogen receptor or human progesterone receptor in any of the conjunctival specimens, in contrast to the strongly positive staining in breast adenocarcinoma controls. Immunocytochemical staining disclosed no evidence for estrogen or progesterone receptors on cells of the ocular surface.
采用单克隆抗体和过氧化物酶-抗过氧化物酶技术，对绝经前和绝经后女性以及男性受试者的新鲜冷冻结膜组织进行雌激素和孕激素受体检测。与乳腺腺癌对照中的强阳性染色不同，在任何结膜标本中，用抗人雌激素受体或抗人孕激素受体单克隆抗体处理的细胞细胞核中均未发现免疫细胞化学染色。免疫细胞化学染色显示眼表细胞上没有雌激素或孕激素受体的证据。
