Dragúňová Jana, Kabát Peter, Koller Ján, Jarabinská Valéria
Department of Burns and Reconstructive Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Cell Tissue Bank. 2012 Aug;13(3):471-8. doi: 10.1007/s10561-011-9275-z. Epub 2011 Aug 17.
Both allogenic and autologous cultured skin cells have been used clinically on burn patients. In vitro cultivation of human keratinocytes has been routinely provided by the Central Tissue Bank in Bratislava since 1996, with an average annual production of around 7,000 cm(2). Keratinocytes have been cultivated using a version of the original by Rheinwald and Green (Cell 6:317-330, 1975) methodology which has been modified over time in our laboratory as we gained more experience with this serial passage system. We have observed that the growth of cultured keratinocytes depends on several important factors, including the timing of skin sample procurement, the method of skin sample procurement, the general condition of the patient, the quality and composition of the culture media and, to a lesser extent, the age of the patient. We aim to share our experience with other cell cultivation facilities.
同种异体和自体培养的皮肤细胞均已在烧伤患者的临床治疗中使用。自1996年以来，布拉迪斯拉发的中央组织库一直常规提供人角质形成细胞的体外培养，年平均产量约为7000平方厘米。角质形成细胞采用的是莱茵瓦尔德和格林（《细胞》6:317 - 330，1975年）最初方法的一个版本进行培养，随着我们在这个连续传代系统中积累了更多经验，该方法在我们实验室中随时间不断改进。我们观察到，培养的角质形成细胞的生长取决于几个重要因素，包括皮肤样本采集的时间、皮肤样本采集的方法、患者的一般状况、培养基的质量和成分，以及在较小程度上患者的年龄。我们旨在与其他细胞培养机构分享我们的经验。
