Ghamari Seyyed-Hadi, Abbasi-Kangevari Mohsen, Tayebi Tahereh, Bahrami Soheyl, Niknejad Hassan
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Student Research Committee, Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Front Bioeng Biotechnol. 2020 Mar 13;8:180. doi: 10.3389/fbioe.2020.00180. eCollection 2020.
Placenta-derived amniotic cells have prominent features for application in regenerative medicine. However, there are still discrepancies in the characterization of human amniotic epithelial and mesenchymal stromal cells. It seems crucial that the characterization of human amniotic membrane cells be investigated to determine whether there are currently discrepancies in their characterization reports. In addition, possible causes for the witnessed discrepancies need to be addressed toward paving the way for further clinical application and safer practices. The objective of this review is to investigate the marker characterization as well as the potential causes of the discrepancies in the previous reports on placenta-derived amniotic epithelial and mesenchymal stromal cells. The current discrepancies could be potentially due to reasons including passage number and epithelial to mesenchymal transition (EMT), cell heterogeneity, isolation protocols and cross-contamination, the region of cell isolation on placental disk, measuring methods, and gestational age.
胎盘来源的羊膜细胞在再生医学应用中具有显著特征。然而，人类羊膜上皮细胞和间充质基质细胞的特征仍存在差异。研究人类羊膜膜细胞的特征以确定其特征报告中目前是否存在差异似乎至关重要。此外，需要解决所观察到的差异的可能原因，为进一步的临床应用和更安全的实践铺平道路。本综述的目的是研究先前关于胎盘来源的羊膜上皮细胞和间充质基质细胞报告中的标志物特征以及差异的潜在原因。目前的差异可能是由于传代次数、上皮-间充质转化（EMT）、细胞异质性、分离方案和交叉污染、胎盘盘上细胞分离的区域、测量方法以及胎龄等原因造成的。
