Xiao Anfeng, Ni Hui, Li Lijun, Cai Huinong
College of Bioengineering, Jimei University, Xiamen 361021, China.
Sheng Wu Gong Cheng Xue Bao. 2011 Apr;27(4):598-605.
A comparative study of batch and repeated batch process was carried out for astaxanthin fermentation of Phaffia rhodozyma to develop a more economical method for astaxanthin industrial production. In shaking flask fermentation, the change of biomass and astaxanthin production was studied to compare the five-day cycle with four-day cycle of repeated batch culture of P. rhodozyma. Astaxanthin production increased at first and then decreased subsequently in seven cycles, yet the yield of astaxanthin in the next six cycles remains higher than that of the first cycle. Comparing the average production of astaxanthin in the seven cycles, four-day cycle performed even better than five-day cycle. Subsequently, a repeated fed-batch process was used in a 5-1 bioreactor. The experimental data showed that biomass and astaxanthin production of the second batch could reach the level of the first batch, no matter that the carbon source was glucose or hydrolysis sugar of starch. This result showed that this strain had good stability, and thus repeated batch and fed-batch process could be applied in astaxanthin fermentation for economical purpose.
对红发夫酵母虾青素发酵的分批培养和重复分批培养进行了比较研究，以开发一种更经济的虾青素工业化生产方法。在摇瓶发酵中，研究了红发夫酵母重复分批培养的五日周期和四日周期生物量及虾青素产量的变化。在七个周期中，虾青素产量先增加后下降，但后六个周期的虾青素产量仍高于第一个周期。比较七个周期虾青素的平均产量，四日周期表现优于五日周期。随后，在5-L生物反应器中采用重复补料分批培养法。实验数据表明，无论碳源是葡萄糖还是淀粉水解糖，第二批培养的生物量和虾青素产量都能达到第一批的水平。该结果表明该菌株具有良好的稳定性，因此重复分批培养和补料分批培养可应用于虾青素发酵以实现经济目的。
