Harauz G, Flannigan D
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
Biochim Biophys Acta. 1990 Apr 19;1038(2):260-7. doi: 10.1016/0167-4838(90)90214-z.
Multivariate statistical analysis and hierarchical ascendant classification techniques have been used to sort electron images of ribosomes from the thermophilic fungus Thermomyces lanuginosus into their characteristic views. Three predominant views were elucidated, called here overlap, non-overlap and top, showing reproducible detail approaching 1.8 nm resolution. The overlap and non-overlap forms of the fungal ribosomes appeared to be similar to those from the eubacterium Escherichia coli, despite differences in rRNA composition. The non-overlap projection predominated for the fungal complexes, suggesting different adsorption properties for ribosomes from the two species. Additionally, the top view has not been previously described for eubacteria. No major morphological differences could be detected between the fungal and eubacterial ribosomes at the resolution achieved in this study, suggesting a strong conservation of tertiary structure of this macromolecular complex despite the evolutionary gap between these two organisms.
多元统计分析和层次上升分类技术已被用于将嗜热真菌嗜热栖热菌的核糖体电子图像分类为其特征视图。阐明了三种主要视图，这里称为重叠、非重叠和顶部视图，显示出接近1.8纳米分辨率的可重复细节。尽管rRNA组成存在差异，但真菌核糖体的重叠和非重叠形式似乎与来自真细菌大肠杆菌的那些相似。真菌复合物中以非重叠投影为主，这表明这两个物种的核糖体具有不同的吸附特性。此外，以前尚未描述过真细菌的顶部视图。在本研究达到的分辨率下，未检测到真菌和真细菌核糖体之间存在主要形态差异，这表明尽管这两种生物之间存在进化差距，但这种大分子复合物的三级结构具有很强的保守性。
