克氏锥虫中的DNA甲基化
DNA methylation in Trypanosoma cruzi.
作者信息
Rojas M V, Galanti N
机构信息
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
出版信息
FEBS Lett. 1990 Apr 9;263(1):113-6. doi: 10.1016/0014-5793(90)80717-w.
PMID:2185032
Abstract
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.
摘要
已发现从原生动物克氏锥虫分离出的DNA含有5-甲基胞嘧啶。用HpaII和MspI限制性内切酶对克氏锥虫DNA进行分析表明，序列-CCGG-未被甲基化。克氏锥虫DNA可能也含有N6-甲基腺嘌呤。本报告首次明确证明了锥虫核DNA中存在甲基化碱基。
Zotero 插件