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凝血因子Xa对融合蛋白的切割。通过可逆酰化消除非特异性切割。

Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation.

作者信息

Wearne S J

机构信息

MRC Laboratory of Molecular Biology, Cambridge, UK.

出版信息

FEBS Lett. 1990 Apr 9;263(1):23-6. doi: 10.1016/0014-5793(90)80696-g.

DOI:10.1016/0014-5793(90)80696-g
PMID:2185034
Abstract

A method is described for the elimination of non-specific cleavage of fusion proteins by factor Xa. Putative non-specific cleavage sites C-terminal to lysyl residues are blocked by reversible acylation by 3,4,5,6-tetrahydrophthalic anhydride prior to cleavage. After cleavage, the acyl groups are removed quantitatively by exposure to slightly acidic conditions. This method employs no harsh reagents or conditions, and may be generally applicable to factor Xa cleavage of fusion proteins.

摘要

描述了一种消除因子Xa对融合蛋白非特异性切割的方法。在切割前，通过3,4,5,6-四氢邻苯二甲酸酐的可逆酰化作用封闭赖氨酸残基C末端的推定非特异性切割位点。切割后，通过暴露于弱酸性条件定量去除酰基。该方法不使用苛刻的试剂或条件，可能普遍适用于因子Xa对融合蛋白的切割。

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