Görlach J, Schmid J
Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich, Switzerland.
Gene. 1996 Apr 17;170(1):145-6. doi: 10.1016/0378-1119(95)00825-x.
The Arg-encoding triplet (AGG) in the recognition sequence Ile-Glu-Gly-Arg for factor Xa can be used to generate a StuI restriction site (AGGCCT) which greatly facilitates the construction of DNA fragments encoding fusion proteins. Following proteolytic cleavage with factor Xa, a protein with the desired N terminus can be obtained.
凝血因子Xa识别序列Ile-Glu-Gly-Arg中的精氨酸编码三联体（AGG）可用于生成一个StuI限制酶切位点（AGGCCT），这极大地便于构建编码融合蛋白的DNA片段。用凝血因子Xa进行蛋白水解切割后，可获得具有所需N端的蛋白质。
