State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University , 1239 Siping Road, Shanghai 200092, China.
Environ Sci Technol. 2011 Oct 1;45(19):8589-95. doi: 10.1021/es2016186. Epub 2011 Sep 9.
After anaerobic dark fermentation of waste activated sludge (WAS) for hydrogen production, there are a large number of organic compounds including protein, polysaccharide, and volatile fatty acids left in the dark fermentation liquid, which can be further bioconverted to hydrogen by photofermentation techniquea. In this study, the enhancement of photofermentative hydrogen produced from WAS dark fermentation liquid by using nano-TiO2 is reported. First, high concentration of NH(4)(+)-N in the dark fermentation liquid was observed to inhibit the photofermentative hydrogen production, and its removal was essential. Then the effect of nano-TiO2 on photofermentative hydrogen generation was investigated, and the addition of 100 mg/L nano-TiO2 increased hydrogen by 46.1%. Finally, the mechanisms for nano-TiO2 improving hydrogen production were investigated. It was found that nano-TiO2 improved the decomposition of protein and polysaccharide to small-molecule organic compounds and promoted the growth of photosynthetic bacteria and the activity of nitrogenase but decreased the H2-uptake hydrogenase activity.
厌氧暗发酵剩余活性污泥（WAS）生产氢气后，在暗发酵液中会留下大量的有机化合物，包括蛋白质、多糖和挥发性脂肪酸，这些有机化合物可以通过光发酵技术进一步转化为氢气。本研究报道了纳米 TiO2 对 WAS 暗发酵液的光发酵产氢的强化作用。首先，高浓度的 NH（4）（+）-N 在暗发酵液中观察到抑制光发酵产氢，去除是必要的。然后考察了纳米 TiO2 对光发酵产氢的影响，添加 100mg/L 纳米 TiO2 可使氢气增加 46.1%。最后，对纳米 TiO2 提高产氢的机理进行了研究。结果表明，纳米 TiO2 提高了蛋白质和多糖分解为小分子有机化合物的能力，并促进了光合细菌的生长和固氮酶的活性，但降低了氢酶的吸氢酶活性。
