Liu Bing-Feng, Xie Guo-Jun, Wang Rui-Qing, Xing De-Feng, Ding Jie, Zhou Xu, Ren Hong-Yu, Ma Chao, Ren Nan-Qi
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090 China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090 China ; Advanced Water Management Centre, The University of Queensland, St. Lucia, QLD 4072 Australia.
Biotechnol Biofuels. 2015 Jan 22;8(1):8. doi: 10.1186/s13068-014-0191-x. eCollection 2015.
Integrating hydrogen-producing bacteria with complementary capabilities, dark-fermentative bacteria (DFB) and photo-fermentative bacteria (PFB), is a promising way to completely recover bioenergy from waste biomass. However, the current coupled models always suffer from complicated pretreatment of the effluent from dark-fermentation or imbalance between dark and photo-fermentation, respectively. In this work, an integrated dark and photo-fermentative reactor (IDPFR) was developed to completely convert an organic substrate into bioenergy.
In the IDPFR, Ethanoligenens harbinese B49 and Rhodopseudomonas faecalis RLD-53 were separated by a membrane into dark and photo chambers, while the acetate produced by E. harbinese B49 in the dark chamber could freely pass through the membrane into the photo chamber and serve as a carbon source for R. faecalis RLD-53. The hydrogen yield increased with increasing working volume of the photo chamber, and reached 3.38 mol H2/mol glucose at the dark-to-photo chamber ratio of 1:4. Hydrogen production by the IDPFR was also significantly affected by phosphate buffer concentration, glucose concentration, and ratio of dark-photo bacteria. The maximum hydrogen yield (4.96 mol H2/mol glucose) was obtained at a phosphate buffer concentration of 20 mmol/L, a glucose concentration of 8 g/L, and a ratio of dark to photo bacteria of 1:20. As the glucose and acetate were used up by E. harbinese B49 and R. faecalis RLD-53, ethanol produced by E. harbinese B49 was the sole end-product in the effluent from the IDPFR, and the ethanol concentration was 36.53 mmol/L with an ethanol yield of 0.82 mol ethanol/mol glucose.
The results indicated that the IDPFR not only circumvented complex pretreatments on the effluent in the two-stage process, but also overcame the imbalance of growth and metabolic rate between DFB and PFB in the co-culture process, and effectively enhanced cooperation between E. harbinense B49 and R. faecalis RLD-53. Moreover, simultaneous hydrogen and ethanol production were achieved by coupling E. harbinese B49 and R. faecalis RLD-53 in the IDPFR. According to stoichiometry, the hydrogen and ethanol production efficiencies were 82.67% and 82.19%, respectively. Therefore, IDPFR was an effective strategy for coupling DFB and PFB to fulfill efficient energy recovery from waste biomass.
将具有互补能力的产氢细菌，即暗发酵细菌（DFB）和光发酵细菌（PFB）整合起来，是从废弃生物质中完全回收生物能源的一种有前景的方法。然而，目前的耦合模型分别总是存在暗发酵出水复杂预处理问题或暗发酵与光发酵之间的不平衡问题。在本研究中，开发了一种集成暗发酵和光发酵反应器（IDPFR），以将有机底物完全转化为生物能源。
在IDPFR中，哈氏乙醇杆菌B49和粪红假单胞菌RLD - 53通过膜被分隔在暗发酵室和光发酵室中，而哈氏乙醇杆菌B49在暗发酵室中产生的乙酸盐可自由透过膜进入光发酵室，并作为粪红假单胞菌RLD - 53的碳源。氢气产量随着光发酵室工作体积的增加而增加，在暗发酵室与光发酵室体积比为1:4时达到3.38 mol H₂/mol葡萄糖。IDPFR产氢还受到磷酸盐缓冲液浓度、葡萄糖浓度和暗发酵菌与光发酵菌比例的显著影响。在磷酸盐缓冲液浓度为20 mmol/L、葡萄糖浓度为8 g/L以及暗发酵菌与光发酵菌比例为1:20时，获得了最大氢气产量（4.96 mol H₂/mol葡萄糖）。随着哈氏乙醇杆菌B49和粪红假单胞菌RLD - 53消耗完葡萄糖和乙酸盐，哈氏乙醇杆菌B49产生的乙醇成为IDPFR流出物中的唯一终产物，乙醇浓度为36.53 mmol/L，乙醇产量为0.82 mol乙醇/mol葡萄糖。
结果表明，IDPFR不仅避免了两阶段过程中对出水的复杂预处理，还克服了共培养过程中DFB和PFB之间生长和代谢速率的不平衡，并有效增强了哈氏乙醇杆菌B49和粪红假单胞菌RLD - 53之间的协同作用。此外，通过在IDPFR中耦合哈氏乙醇杆菌B49和粪红假单胞菌RLD - 53实现了同时产氢和产乙醇。根据化学计量学，氢气和乙醇的生产效率分别为82.67%和82.19%。因此，IDPFR是耦合DFB和PFB以实现从废弃生物质中高效回收能量的有效策略。
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