Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Genomics. 2011 Nov;98(5):370-5. doi: 10.1016/j.ygeno.2011.08.001. Epub 2011 Aug 7.
The anaerobic thermophilic bacterial genus Dictyoglomus is characterized by the ability to produce useful enzymes such as amylase, mannanase, and xylanase. Despite the significance, the phylogenetic position of Dictyoglomus has not yet been clarified, since it exhibits ambiguous phylogenetic positions in a single gene sequence comparison-based analysis. The number of substitutions at the diverging point of Dictyoglomus is insufficient to show the relationships in a single gene comparison-based analysis. Hence, we studied its evolutionary trait based on whole-genome comparison. Both gene content and orthologous protein sequence comparisons indicated that Dictyoglomus is most closely related to the phylum Thermotogae and it forms a monophyletic group with Coprothermobacter proteolyticus (a constituent of the phylum Firmicutes) and Thermotogae. Our findings indicate that C. proteolyticus does not belong to the phylum Firmicutes and that the phylum Dictyoglomi is not closely related to either the phylum Firmicutes or Synergistetes but to the phylum Thermotogae.
厌氧嗜热细菌属 Dictyoglomus 的特征是能够产生有用的酶，如淀粉酶、甘露聚糖酶和木聚糖酶。尽管意义重大，但 Dictyoglomus 的系统发育位置尚未得到澄清，因为在基于单一基因序列比较的分析中，它表现出模糊的系统发育位置。在 Dictyoglomus 的分歧点发生的替换数量不足以在单一基因比较分析中显示关系。因此，我们基于全基因组比较研究了它的进化特征。基因内容和直系同源蛋白序列比较都表明，Dictyoglomus 与热球菌门关系最密切，它与 Coprothermobacter proteolyticus（厚壁菌门的一个组成部分）和热球菌门形成一个单系群。我们的研究结果表明，C. proteolyticus 不属于厚壁菌门，而 Dictyoglomi 门与厚壁菌门或互养菌门都没有密切关系，而是与热球菌门关系密切。
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