Brumm Phillip J, Gowda Krishne, Robb Frank T, Mead David A
C5-6 Technologies LLCFitchburg, WI, USA; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-MadisonMadison, WI, USA.
DOE Great Lakes Bioenergy Research Center, University of Wisconsin-MadisonMadison, WI, USA; Lucigen CorporationMiddleton, WI, USA.
Front Microbiol. 2016 Dec 20;7:1979. doi: 10.3389/fmicb.2016.01979. eCollection 2016.
Here we report the complete genome sequence of the chemoorganotrophic, extremely thermophilic bacterium, , which is a Gram negative, strictly anaerobic bacterium. and together form the phylum. The two genomes are highly syntenic, and both are distantly related to spp. is able to grow on a wide variety of polysaccharide substrates due to significant genomic commitment to glycosyl hydrolases, 16 of which were cloned and expressed in our study. The GH5, GH10, and GH42 enzymes characterized in this study suggest that can utilize most plant-based polysaccharides except crystalline cellulose. The DNA polymerase I enzyme was also expressed and characterized. The pure enzyme showed improved amplification of long PCR targets compared to Taq polymerase. The genome contains a full complement of DNA modifying enzymes, and an unusually high copy number (4) of a new, ancestral family of polB type nucleotidyltransferases designated as MNT (minimal nucleotidyltransferases). Considering its optimal growth at 72°C, has an anomalously low G+C content of 39.9% that may account for the presence of reverse gyrase, usually associated with hyperthermophiles.
在此，我们报道了化能有机营养型极端嗜热细菌的完整基因组序列，该细菌为革兰氏阴性、严格厌氧细菌。与共同构成了门。这两个基因组高度共线，且均与种属关系较远。由于对糖基水解酶有大量的基因组投入，能够在多种多糖底物上生长，本研究中克隆并表达了其中16种。本研究中表征的GH5、GH10和GH42酶表明，除了结晶纤维素外，能够利用大多数植物来源的多糖。还表达并表征了DNA聚合酶I酶。与Taq聚合酶相比，纯酶对长PCR靶点的扩增效果更好。该基因组包含一套完整的DNA修饰酶，以及一个新的、祖先型的polB型核苷酸转移酶家族（命名为MNT，即最小核苷酸转移酶）的异常高拷贝数（4个）。考虑到其在72°C时的最佳生长状态，的G+C含量异常低，为39.9%，这可能解释了通常与超嗜热菌相关的反向回旋酶的存在。
