Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
J Pharm Biomed Anal. 2011 Dec 15;56(5):911-5. doi: 10.1016/j.jpba.2011.07.037. Epub 2011 Jul 30.
Red Sea sponges offer a potential for production of novel drugs and prototypes. The genus Negombata is a type of sponges abundant in the Red Sea. This sponge produces latrunculins that have well documented antitumor activity in addition to antimicrobial and antiviral effects. The identification of Negombata species is based on morphology and microscopical examination of megascleres of spicules. However, these criteria have proven to be unreliable for identification. Therefore, this study was established to test the accuracy of the spicules based taxonomy against molecular and protein profiles for the two species of Negombata: N. magnifica and N. corticata. About 700 bp of cytochrome c oxidase I gene was sequenced from the tissues of the two Negombata species. Additionally total proteins were extracted from Negombata samples collected from different locations during different seasons and separated by denaturing polyacrylamide gel electrophoresis. Characteristic different protein profiles were obtained for both species. The data obtained from cytochrome c oxidase I gene sequencing and protein profiles can reliably differentiate between different species of Negombata in the Red Sea.
红海海绵为新型药物和原型的生产提供了潜力。Negombata 属是红海丰富的海绵种类之一。这种海绵产生的拉曲菌素具有良好的抗肿瘤活性，此外还有抗菌和抗病毒作用。Negombata 属的鉴定基于形态学和对大骨针的 megascleres 的显微镜检查。然而，这些标准已被证明对鉴定不可靠。因此，本研究旨在测试基于骨针的分类学对分子和蛋白质图谱的准确性，以确定两种 Negombata 物种：N. magnifica 和 N. corticata。从两种 Negombata 物种的组织中测序了约 700 bp 的细胞色素 c 氧化酶 I 基因。此外，还从不同季节在不同地点采集的 Negombata 样本中提取了总蛋白，并通过变性聚丙烯酰胺凝胶电泳进行分离。两种物种均获得了特征性不同的蛋白质图谱。细胞色素 c 氧化酶 I 基因测序和蛋白质图谱获得的数据可可靠地区分红海不同的 Negombata 物种。
