Department of Microbiology & Immunology, College of Pharmacy, Suez Canal University, Ismailia, Egypt; Department of Microbiology & Immunology, College of Pharmacy, Sinai University, Ismailia, Egypt.
Department of Microbiology & Immunology, College of Pharmacy, Suez Canal University, Ismailia, Egypt.
Mar Genomics. 2022 Feb;61:100920. doi: 10.1016/j.margen.2021.100920. Epub 2021 Dec 29.
Red Sea marine sponges (phylum Porifera) and associated microorganisms harbor a wide range of microorganisms, which are considered an essential source of bioactive products. In this study, we screened both the crude extracts of Red Sea marine sponges and their associated bacterial extract for antimicrobial activity and antiviral activity. Molecular characterization of bioactive producers was performed using16S rRNA sequencing, in addition to metagenomic analysis of three representative sponges utilizing the 16S rRNA gene V3-V4 region sequencing in two different seasons. Twelve samples were collected from five different sponge species by scuba diving, and all the crude extracts of sponges showed antimicrobial activity except Negombata corticata. Moreover, 84 out of 110 bacterial isolates extracts demonstrated antimicrobial activity against at least one tested microorganism. These results revealed the bioactivity and biodiversity of the Red Sea marine invertebrates-associated bacteria. It was found that the bioactive isolates belong to several bacterial groups. The bacterial communities of Negombata magnifica, Negombata corticata, and Siphonochalina siphonella were shown with great diversity and differences in the bacterial percentage, diversity, and unique community composition at different seasons in each sponge species. Unique microenvironment for each sponge species may be linked to the production of specific bioactive product.
红海海洋海绵（多孔动物门）及其相关微生物群中蕴藏着大量的微生物，这些微生物被认为是生物活性产物的重要来源。在本研究中，我们对红海海洋海绵的粗提取物及其相关细菌提取物进行了筛选，以检测其抗微生物活性和抗病毒活性。通过 16S rRNA 测序对生物活性产生菌进行了分子特征分析，此外，还利用 16S rRNA 基因 V3-V4 区测序对三种具有代表性的海绵进行了宏基因组分析，分别在两个不同的季节进行。通过水肺潜水采集了来自五个不同海绵物种的 12 个样本，除了 Negombata corticata 外，所有海绵的粗提取物均显示出抗微生物活性。此外，110 个细菌分离物提取物中有 84 个对至少一种测试微生物具有抗微生物活性。这些结果揭示了红海海洋无脊椎动物相关细菌的生物活性和生物多样性。结果发现，生物活性分离物属于几个细菌群。在不同季节中，Negombata magnifica、Negombata corticata 和 Siphonochalina siphonella 的细菌群落表现出很大的多样性，细菌百分比、多样性和独特的群落组成存在差异。每个海绵物种独特的微环境可能与特定生物活性产物的产生有关。
