Center for Bioproducts and Bioenergy, Bioproducts Sciences and Engineering Laboratory, Washington State University, Richland, WA 99354-1671, USA.
Bioresour Technol. 2011 Oct;102(20):9718-22. doi: 10.1016/j.biortech.2011.07.073. Epub 2011 Jul 28.
Endophytic fungi belonging to the genus Gliocladium are able to degrade plant cellulose and synthesize complex hydrocarbons under microaerophilic conditions. These fungi could thus be used to produce biofuels from cellulosics without the need for hydrolytic pretreatments. Gas chromatography-mass spectrometry-solid-phase micro-extraction (GC-MS-SPME) of head space gases from Gliocladium cultures demonstrated the production of C(6)-C(19) hydrocarbons including hexane, benzene, heptane, 3,4-dimethyl hexane, 1-octene, m-xylene, 3-methyl nonane, dodecane, tridecane, hexadecane and nonadecane directly from the cellulosic biomass. Hydrocarbon production was 100-fold higher in co-cultures of Gliocladium and Escherichia coli than in pure Gliocladium cultures. The dry mycelia weight is stable at stationary period in co-culture condition which may lead to synthesize more hydrocarbons. These fungi could potentially be developed into cost-effective biocatalysts for production of biofuels.
属于木霉属的内生真菌能够在微氧条件下降解植物纤维素并合成复杂的碳氢化合物。因此，这些真菌可以用来生产纤维素生物燃料，而无需进行水解预处理。通过顶空气体的气相色谱-质谱-固相微萃取（GC-MS-SPME）分析木霉属培养物，证明了包括己烷、苯、庚烷、3,4-二甲基己烷、1-辛烯、间二甲苯、3-甲基壬烷、十二烷、十三烷、十六烷和十九烷在内的 C(6)-C(19)碳氢化合物的直接从纤维素生物质中生产。与纯木霉属培养物相比，木霉属和大肠杆菌的共培养物中的碳氢化合物产量高出 100 倍。在共培养条件下，干菌丝体重量在静止期保持稳定，这可能导致合成更多的碳氢化合物。这些真菌有可能被开发成为具有成本效益的生物催化剂，用于生产生物燃料。
