Sharma Deeksha, Pramanik Avijit, Agrawal Pavan Kumar
Department of Biotechnology, G. B. Pant Engineering College, Ghurdauri, Pauri, Uttarakhand, 246194, India.
Department of Microbiology, Central University of Haryana, Mahendergarh, Haryana, 123031, India.
3 Biotech. 2016 Dec;6(2):210. doi: 10.1007/s13205-016-0518-3. Epub 2016 Sep 29.
Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp. showed MIC of 6.25 mg/mL for S. typhimurium and S. aureus which showed its efficacy as a potent antimicrobial. The phytochemical screening revealed the existence of a diverse group of secondary metabolites in the crude extracts of the endophytic fungi that resembled those in the host plant extracts. On the basis of phenotypic characteristics and rDNA sequencing of the ITS region of the endophyte was identified as P. neglecta which turned out to be a promising source of bioactive compounds. There is little known about endophytes from C. torulosa D.Don. In this paper we studied in detail the identification of isolated endophytic fungi P. neglecta from C. torulosa D.Don and characterization of its active metabolite compounds. The partially purified second fraction (PPF) extracted from the fungal culture supernatant was subjected to gas chromatography followed by mass spectrometry which revealed the presence of many phytochemicals. These results indicate that endophytic fungi P. neglecta isolated from medicinal plants could be a potential source for bioactive compounds and may find potential use in pharmaceutical industry.
从喜马拉雅柏木中分离出6种内生真菌，并对其进行了表型和基因型鉴定。进一步培养真菌培养物，并用甲醇和乙酸乙酯两种有机溶剂进行提取。采用琼脂孔扩散法对大肠杆菌、鼠伤寒沙门氏菌、枯草芽孢杆菌和金黄色葡萄球菌等人类病原体进行筛选。分离出的拟盘多毛孢属菌株显示出显著的抗菌活性。拟盘多毛孢属的粗甲醇提取物和乙酸乙酯提取物对鼠伤寒沙门氏菌和金黄色葡萄球菌的最低抑菌浓度为6.25mg/mL，显示出其作为强效抗菌剂的功效。植物化学筛选表明，内生真菌粗提物中存在多种次生代谢产物，与宿主植物提取物中的次生代谢产物相似。根据表型特征和内生菌ITS区域的rDNA测序，鉴定为疏花拟盘多毛孢，结果表明它是一种很有前景的生物活性化合物来源。关于喜马拉雅柏木内生菌的了解很少。在本文中，我们详细研究了从喜马拉雅柏木中分离出的内生真菌疏花拟盘多毛孢的鉴定及其活性代谢产物的表征。从真菌培养上清液中提取的部分纯化的第二馏分（PPF）进行气相色谱分析，然后进行质谱分析，结果显示存在许多植物化学物质。这些结果表明，从药用植物中分离出的内生真菌疏花拟盘多毛孢可能是生物活性化合物的潜在来源，并可能在制药工业中找到潜在用途。
