大肠杆菌mutT1突变基因缺陷的特征分析
Characterization of the defect in the Escherichia coli mutT1 mutator gene.
作者信息
Bhatnagar S K, Bullions L C, Lew G, Bessman M J
机构信息
McCollum-Pratt Institute, Johns Hopkins University, Baltimore, Maryland 21218.
出版信息
J Bacteriol. 1990 May;172(5):2802-3. doi: 10.1128/jb.172.5.2802-2803.1990.
Abstract
With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.
摘要
用由野生型基因构建的探针，分离出一个包含整个mutT1诱变基因的DNA片段，并将其克隆到pUC18中。核苷酸序列分析表明，诱变缺陷很可能是由于一个IS1插入到野生型基因中。
Zotero 插件