高效酰化生物素化的无黏附细胞用于细胞微阵列的形成。
Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays.
机构信息
Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita-shi, Osaka 564-8680, Japan.
出版信息
Chem Commun (Camb). 2011 Oct 7;47(37):10329-31. doi: 10.1039/c1cc12948a. Epub 2011 Aug 19.
PMID:21853201
Abstract
In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.
摘要
为了获得与人早幼粒细胞白血病细胞（HL-60）形成的细胞微阵列，通过巯基-烯反应用巯基封端的 4 臂聚乙二醇（PEG（4）10K-SH）对甲丙烯酰基功能化的 HL-60 细胞进行生物素化。生物素化的 HL-60 细胞以高活力选择性地粘附在亲和素图案化表面上。
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