Dipartimento di Sanità Pubblica e Zootecnia - Facoltà di Medicina Veterinaria, Università degli Studi di Bari Aldo Moro, Valenzano, Bari, Italy.
Curr Microbiol. 2011 Nov;63(5):416-9. doi: 10.1007/s00284-011-9996-z. Epub 2011 Aug 19.
Given the considerable economic loss to beekeepers worldwide and the possible public health implications related to the presence of antibiotics in honey, an American Foulbrood (AFB) monitoring/prevention program for Paenibacillus larvae is regarded as essential. This study investigates the occurrence and distribution of P. larvae genotypes in honey and brood combs from Apulia (Italy). Genotyping of P. larvae isolates using ERIC-PCR generated a total of four different ERIC banding patterns (ERIC-A, ERIC-B, ERIC-C, ERIC-D), including fragments ranging from 200 to 3000 bp. Considering that the genotype has an influence on P. larvae infections and multi-genotype infections of colonies or apiaries may increase the complexity of P. larvae infections by influencing the type and speed of the development of clinical symptoms, the findings of the present study could be helpful for training veterinarians, bee inspector's extension staff, and beekeepers, thus improving the detection of AFB infections in the field.
鉴于全世界养蜂人遭受的巨大经济损失，以及蜂蜜中抗生素存在可能对公共卫生造成的影响，对美洲幼虫腐臭病（AFB）进行 Paenibacillus larvae 监测/预防计划被认为是至关重要的。本研究调查了普利亚（意大利）地区蜂蜜和巢脾中幼虫基因型的发生和分布。使用 ERIC-PCR 对 P. larvae 分离株进行基因分型总共产生了四种不同的 ERIC 带型（ERIC-A、ERIC-B、ERIC-C、ERIC-D），包括 200 到 3000bp 之间的片段。考虑到基因型对 P. larvae 感染有影响，并且通过影响临床症状的发展类型和速度，多基因型感染蜂群或蜂场可能会增加 P. larvae 感染的复杂性，本研究的结果可能有助于培训兽医、蜜蜂检查员推广人员和养蜂人，从而提高现场检测 AFB 感染的能力。
