Dingman Douglas W
Department of Entomology, The Connecticut Agricultural Experiment Station, 123 Huntington Street, P. O. Box 1106, New Haven, CT, 06504, USA,
Arch Microbiol. 2015 Aug;197(6):789-95. doi: 10.1007/s00203-015-1113-4. Epub 2015 May 1.
Ninety-six strains of Paenibacillus larvae, causative agent of American foulbrood in honey bee (Apis mellifera) larvae, collected from Connecticut, USA (CT), honey bees, and 12 P. larvae strains not from CT, were genotyped via ERIC-PCR and XbaI-RFLP analysis. All CT-isolates, five strains isolated in South America, three strains from North America (not CT), and one strain isolated in Australia grouped into the ERIC I genotype. Three P. larvae formerly subsp. pulvifaciens strains grouped into ERIC III and IV genotypes. XbaI-RFLP genotyping showed three genotypes within the CT-isolates, and two were identified as XbaI-RFLP Type I and III. The third XbaI-RFLP genotype (Type Ib) represented one of four new XbaI-RFLP genotypes identified. Comparison of genotype results for the P. larvae strains tested was used to develop a correlation between ERIC-PCR genotyping and XbaI-RFLP genotyping. Sixteen CT-isolates were tetracycline-resistant and demonstrated PCR amplification using oligonucleotide primers for tetL. All 16 isolates grouped within XbaI-RFLP Type Ib, suggesting limited introduction of a tetracycline-resistant strain into CT.
从美国康涅狄格州（CT）的蜜蜂（意大利蜜蜂）中收集了96株幼虫芽孢杆菌（美洲幼虫腐臭病的病原体），并对12株非CT来源的幼虫芽孢杆菌菌株进行了ERIC-PCR和XbaI-RFLP分析基因分型。所有CT分离株、在南美洲分离的5株、来自北美（非CT）的3株以及在澳大利亚分离的1株均归为ERIC I基因型。3株以前的粉状幼虫亚种菌株归为ERIC III和IV基因型。XbaI-RFLP基因分型显示CT分离株中有三种基因型，其中两种被鉴定为XbaI-RFLP I型和III型。第三种XbaI-RFLP基因型（Ib型）代表鉴定出的四种新XbaI-RFLP基因型之一。通过比较所测试的幼虫芽孢杆菌菌株的基因型结果，建立了ERIC-PCR基因分型与XbaI-RFLP基因分型之间的相关性。16株CT分离株对四环素耐药，并且使用tetL的寡核苷酸引物进行PCR扩增。所有16株分离株均归为XbaI-RFLP Ib型，这表明四环素耐药菌株向CT的引入有限。
