Institut für Chemie, Humboldt Universität zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany.
Chembiochem. 2011 Oct 17;12(15):2302-5. doi: 10.1002/cbic.201100445. Epub 2011 Aug 18.
Without labeling RNA: A new rapid assay for micro-RNA maturation was developed. The assay depends on the cleavage of unmodified pre-miRNAs and the subsequent amplification of the mature miRNA by rolling circle amplification (see accompanying scheme).
无标记 RNA:开发了一种新的 microRNA 成熟的快速检测方法。该检测方法依赖于未修饰的 pre-miRNA 的切割,以及随后通过滚环扩增对成熟 miRNA 的扩增(参见附图)。