Institute for Chemistry, Humboldt Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany.
Chembiochem. 2020 Mar 16;21(6):793-796. doi: 10.1002/cbic.201900514. Epub 2019 Nov 27.
Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy assay for the detection of natural circRNA, based on rolling-circle amplification (RCA). This technique does not require the use of fluorescently labeled RNA or DNA and can specifically detect circular RNA in the presence of a 1000-fold excess of the same linear RNA. Only standard devices such as (quantitative) PCR cyclers and gel electrophoresis are used.
环状 RNA（circRNAs）代表了一类通常是非编码转录本的新型分子，其功能在很大程度上尚不清楚。它们的研究受到传统分析方法不适用的阻碍。本文描述了一种基于滚环扩增（RCA）的快速简便的天然环状 RNA 检测方法。该技术不需要使用荧光标记的 RNA 或 DNA，并且可以在存在 1000 倍过量相同线性 RNA 的情况下特异性检测环状 RNA。仅使用标准设备，如（定量）PCR 循环仪和凝胶电泳。
