Wertel Iwona, Polak Grzegorz, Tarkowski Rafał, Kotarska Maria
Department of Oncological Gynaecology and Gynaecology, Medical University, Lublin, Poland.
Ginekol Pol. 2011 Jun;82(6):414-20.
The aim of the study was to evaluate IL-10 and TGF-beta levels in the peritoneal fluid (PF) and plasma of patients with ovarian cancer (n=104), serous cyst (n=32) or normal controls (n=20). IL-10 and TGF-beta levels were correlated to myeloid (M) and lymphoid (L) dendritic cells (DC).
IL- 10 and TGF-beta concentrations were evaluated using the enzyme linked immunosorbent assay (ELISA). The percentage of DC in mononuclear cells was quantified by using flow cytometry.
The PF and plasma IL-10 concentrations were significantly higher in epithelial ovarian cancer (EOC) patients when compared to the women with serous cyst (the reference group). Plasma levels of IL-10 were elevated in EOC patients in comparison with the reference and control groups. There were significant differences in the PF and plasma IL-10 levels with respect to tumor stage, grade and histology Significant negative correlations were found between the plasma IL-10 levels, MDC and LDC in the peripheral blood. TGF-beta levels were detected in PF of all EOC patients and were significantly lower when compared with plasma. Plasma levels of TGF-beta were elevated in EOC patients compared with the control group. No significant differences in the PF and plasma TGF-beta levels were noted between EOC patients and the reference group. The authors did not find a correlation between the plasma and PF TGF-beta levels and the percentage of MDC and LDC.
IL-10 production in EOC patients depends on the tumor stage, grade and histological type of the tumor cells. IL-10 may have impact on the percentage of dendritic cells in EOC patients.
本研究旨在评估卵巢癌患者（n = 104）、浆液性囊肿患者（n = 32）或正常对照者（n = 20）的腹腔液（PF）和血浆中白细胞介素-10（IL-10）及转化生长因子-β（TGF-β）水平。IL-10和TGF-β水平与髓样（M）和淋巴样（L）树突状细胞（DC）相关。
采用酶联免疫吸附测定（ELISA）评估IL-10和TGF-β浓度。通过流式细胞术定量单核细胞中DC的百分比。
与浆液性囊肿患者（参照组）相比，上皮性卵巢癌（EOC）患者的PF和血浆IL-10浓度显著更高。与参照组和对照组相比，EOC患者的血浆IL-10水平升高。PF和血浆IL-10水平在肿瘤分期、分级和组织学方面存在显著差异。外周血中血浆IL-10水平与MDC和LDC之间存在显著负相关。所有EOC患者的PF中均检测到TGF-β水平，与血浆相比显著更低。与对照组相比，EOC患者的血浆TGF-β水平升高。EOC患者与参照组之间的PF和血浆TGF-β水平未发现显著差异。作者未发现血浆和PF中TGF-β水平与MDC和LDC百分比之间存在相关性。
EOC患者中IL-10的产生取决于肿瘤细胞的肿瘤分期、分级和组织学类型。IL-10可能对EOC患者树突状细胞的百分比有影响。
