Wertel Iwona, Tarkowski Rafal, Bednarek Wieslawa, Kotarski Jan
1st Department of Gynaecology, University School of Medicine, Lublin, Poland.
Front Biosci (Elite Ed). 2011 Jan 1;3(1):227-32. doi: 10.2741/e237.
This study was undertaken to evaluate RANTES levels in the peritoneal fluid (PF) and plasma of patients with ovarian cancer (n=73), serous cystadenoma (n=32) or normal controls (n=9). RANTES levels were correlated to myeloid and lymphoid dendritic cells (DCs). RANTES levels were evaluated using the ELISA assay. DCs were quantified using flow cytometry. The PF and plasma RANTES concentrations were elevated in the ovarian cancer (OVC) patients when compared to the patients with benign tumor (the reference group). Plasma levels of RANTES were higher in OVC patients compared with the reference group and with the controls. There were no significant differences in the plasma RANTES levels based on tumor stage, grade or histology. Women with serous cystadenocarcinoma, clear cell carcinoma and endometrioid cystadenocarcinoma had significantly higher PF RANTES levels than patients with undifferentiated carcinoma. Women with clear cell carcinoma and patients with endometrioid cystadenocarcinoma had higher PF RANTES levels than women with mucinous cystadenocarcinoma. We concluded that RANTES production in the peritoneal cavities of OVC patients depends on the histological type of the tumor cells.
本研究旨在评估卵巢癌患者（n = 73）、浆液性囊腺瘤患者（n = 32）或正常对照者（n = 9）腹腔液（PF）和血浆中调节激活正常T细胞表达和分泌的趋化因子（RANTES）水平。RANTES水平与髓样和淋巴样树突状细胞（DCs）相关。使用酶联免疫吸附测定（ELISA）法评估RANTES水平。使用流式细胞术对DCs进行定量分析。与良性肿瘤患者（参照组）相比，卵巢癌（OVC）患者的PF和血浆RANTES浓度升高。与参照组和对照组相比，OVC患者的血浆RANTES水平更高。基于肿瘤分期、分级或组织学，血浆RANTES水平无显著差异。浆液性囊腺癌、透明细胞癌和子宫内膜样囊腺癌患者的PF RANTES水平显著高于未分化癌患者。透明细胞癌患者和子宫内膜样囊腺癌患者的PF RANTES水平高于黏液性囊腺癌患者。我们得出结论，OVC患者腹腔内RANTES的产生取决于肿瘤细胞的组织学类型。
