Department of BioMedical Sciences, Women Research Center, Alzahra University, Tehran, Iran.
Can J Microbiol. 2011 Sep;57(9):730-4. doi: 10.1139/w11-060. Epub 2011 Aug 22.
Astaxanthin is a carotenoid pigment responsible for the red color of the flesh of many marine animals. There is an increasing interest in the use of astaxanthin in aquaculture, chemical, pharmaceutical, and alimentary industries. Phaffia rhodozyma has been identified as the best biological source of astaxanthin. Mutagenesis was carried out using different doses of gamma irradiation (1.0, 2.0, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0 kGy), and 10 mutant colonies (Gam1-Gam10) were obtained. Highly pigmented mutant strains produced astaxanthin at approximately 15 887.5 µg/L dry mass of yeast, whereas the parental strain produced it at 1061.64 µg/g dry mass of yeast. In the thin-layer chromatography analysis, P. rhodozyma JH-82 and Gam1 mutant strain produced the same retention factor (R(f)) values, but Gam1 showed a higher astaxanthin content than JH-82.
虾青素是一种类胡萝卜素色素，使许多海洋动物的肉呈现红色。虾青素在水产养殖、化学、制药和食品工业中的应用越来越受到关注。雨生红球藻已被确定为虾青素的最佳生物来源。使用不同剂量的γ射线（1.0、2.0、3.0、3.5、4.0、4.5、5.0、5.5、6.0 和 7.0 kGy）进行了诱变，获得了 10 个突变体菌落（Gam1-Gam10）。高度着色的突变株在酵母干质量中产生约 15,887.5 µg/L 的虾青素，而亲本菌株在酵母干质量中产生 1,061.64 µg/g 的虾青素。在薄层层析分析中，P. rhodozyma JH-82 和 Gam1 突变株产生相同的保留因子（R(f)）值，但 Gam1 的虾青素含量高于 JH-82。
