Cemagref, UR GERE, Rennes, France.
J Appl Microbiol. 2011 Nov;111(5):1159-75. doi: 10.1111/j.1365-2672.2011.05131.x. Epub 2011 Sep 14.
The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen.
River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20°C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20°C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10·5, 8·1 and 17·2 days, respectively.
Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters.
The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.
本研究旨在评估源自猪粪的拟杆菌-普雷沃氏菌和芽孢杆菌-链球菌-乳杆菌种群的动态变化，以及根据温度和氧气，这些菌群中与猪相关的标记物的持久性。
将河水接种猪粪，并在微需氧和需氧条件下于 4 和 20°C 下孵育，持续 43 天。通过毛细管电泳-单链构象多态性分析细菌种群的多样性。通过实时 PCR 测量与猪相关的标记物的持久性，并与大肠杆菌和肠球菌的存活情况进行比较。通过估计产生 1 个对数减少所需的时间（T90）来表征衰减。在有氧条件下，20°C 时观察到最大的变化，导致细菌种群多样性降低，以及 Pig-1-Bac、Pig-2-Bac 和嗜酸乳杆菌标记物的浓度降低，T90 分别为 10.5、8.1 和 17.2 天。
氧气和温度被发现对河流中与猪相关的标记物的持久性有综合影响。
Pig-1-Bac、Pig-2-Bac 和 Lact.amylovorus 标记物的持久性特征，加上它们的高特异性和灵敏度，支持它们作为识别河水中猪粪污染的相关标记物的使用。
