血清对氧磷酶 3 浓度与慢性肝病患者肝损伤严重程度相关。
Serum paraoxonase-3 concentration is associated with the severity of hepatic impairment in patients with chronic liver disease.
机构信息
Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, 43201 Reus, Catalonia, Spain.
出版信息
Clin Biochem. 2011 Nov;44(16):1320-4. doi: 10.1016/j.clinbiochem.2011.08.003. Epub 2011 Aug 10.
OBJECTIVES
Research on paraoxonase-3 (PON3) has been hampered by the lack of methods for measurement. This is a pilot study aimed at exploring whether chronic liver impairment is associated with changes in serum PON3 concentrations, and to know whether this measurement may provide useful information to investigate this derangement.
DESIGN AND METHODS
We studied 110 patients with chronic liver disease (21 minimal changes, 79 chronic hepatitis, 10 cirrhosis) and 356 healthy volunteers. Serum PON3 concentration was determined by ELISA using polyclonal antibodies generated against a synthetic peptide with a sequence specific to PON3.
RESULTS
Serum PON3 concentrations were increased in patients with chronic hepatitis or cirrhosis and showed significant direct correlations with the degree of periportal abnormalities including fibrosis, and with serum FAS (a marker of antiapoptosis) concentrations.
CONCLUSION
These results suggest that PON3 may play a hepatoprotective role against histological alterations and hepatic cell apoptosis leading to liver disease.
目的
对人对氧磷酶 3（PON3）的研究受到缺乏测量方法的阻碍。这是一项旨在探索慢性肝损伤是否与血清 PON3 浓度变化相关的初步研究，以了解该测量是否可能提供有用的信息来研究这种紊乱。
设计和方法
我们研究了 110 例慢性肝病患者（21 例微小变化，79 例慢性肝炎，10 例肝硬化）和 356 例健康志愿者。使用针对 PON3 特异性合成肽的多克隆抗体，通过 ELISA 法测定血清 PON3 浓度。
结果
慢性肝炎或肝硬化患者的血清 PON3 浓度升高，与包括纤维化在内的门脉周围异常的程度以及血清 FAS（抗细胞凋亡的标志物）浓度呈显著直接相关。
结论
这些结果表明，PON3 可能在对抗导致肝脏疾病的组织学改变和肝细胞凋亡方面发挥肝保护作用。
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