苦棟和诃子中具有杀软体动物活性成分对尖音库蚊神经组织中某些酶的抑制动力学。
Inhibition kinetics of certain enzymes in the nervous tissue of vector snail Lymnaea acuminata by active molluscicidal components of Sapindus mukorossi and Terminalia chebula.
机构信息
Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, UP, India.
出版信息
Chemosphere. 2011 Oct;85(6):1095-100. doi: 10.1016/j.chemosphere.2011.07.041. Epub 2011 Aug 19.
Effect of active molluscicidal components of Sapindus mukorossi and Terminalia chebula on the acetylcholinesterase (AChE), acid and alkaline phosphatase (ACP/ALP) activity in the nervous tissue of freshwater snail Lymnaea acuminata were studied. In vivo and in vitro exposure of saponin (active component of S. mukorossi pericarp) and tannic acid (active component of T. chebula) significantly inhibited the AChE, ACP and ALP activity in the nervous tissue of L. acuminata. The inhibition kinetics of these enzymes indicate that saponin and tannic acid caused competitive and competitive-non-competitive inhibition of AChE, respectively. Saponin also caused competitive and competitive-non-competitive inhibition of ACP and ALP, respectively, whereas tannic acid caused competitive-non-competitive inhibition of ACP and ALP. Thus the inhibition of AChE, ACP and ALP by saponin and tannic acid in the nervous tissue of L. acuminata may be the cause of molluscicidal activity of S. mukorossi and T. chebula.
研究了无患子和诃子的活性杀螺成分对淡水螺 Lymnaea acuminata 神经组织中乙酰胆碱酯酶（AChE）、酸性和碱性磷酸酶（ACP/ALP）活性的影响。在体内和体外暴露于皂素（无患子果皮的活性成分）和鞣酸（诃子的活性成分）显著抑制了 Lymnaea acuminata 神经组织中的 AChE、ACP 和 ALP 活性。这些酶的抑制动力学表明，皂素和鞣酸分别导致 AChE 的竞争性和竞争性非竞争性抑制。皂素还分别导致 ACP 和 ALP 的竞争性和竞争性非竞争性抑制，而鞣酸导致 ACP 和 ALP 的竞争性非竞争性抑制。因此，皂素和鞣酸在 Lymnaea acuminata 神经组织中对 AChE、ACP 和 ALP 的抑制可能是无患子和诃子杀螺活性的原因。
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