Department of Life Science, College of Life Science, National Taiwan University, Taipei, Taiwan.
Food Chem Toxicol. 2011 Nov;49(11):2890-8. doi: 10.1016/j.fct.2011.08.007. Epub 2011 Aug 12.
Mushroom β-glucan, a polymer of β-(1,3/1,6)-glucan, has been claimed for its health benefits. The objective of this study was to assess the safety in-use of mushroom β-glucan as dietary supplement and food ingredient. Hence, a subchronic toxicity and mutagenicity studies were conducted. In the subchronic toxicity study, Sprague Dawley rats (12/sex/group) were administered (gavage) mushroom β-glucan at dose levels of 0, 500, 1000 and 2000 mg/kg body weight (bw)/day for 90 days. As compared to control group, administration of β-glucan did not result in any toxicologically significant treatment-related changes in clinical observations, ophthalmic examinations, body weights, body weight gains, feed consumption, and organ weights. No adverse effects of the β-glucan on the hematology, serum chemistry parameters, urinalysis or terminal necropsy (gross or histopathology findings) were noted. The results of mutagenicity studies as evaluated by gene mutations in Salmonella typhimurium, in vitro chromosome aberrations and in vivo micronucleus test in mouse did not reveal any genotoxicity of β-glucan. Based on the subchronic study, the no observed-adverse-effect level (NOAEL) for mushroom β-glucan was determined as 2000 mg/kgbw/day, the highest dose tested.
蘑菇 β-葡聚糖是一种 β-(1,3/1,6)-葡聚糖的聚合物，据称具有健康益处。本研究的目的是评估蘑菇 β-葡聚糖作为膳食补充剂和食品成分的使用安全性。因此，进行了亚慢性毒性和致突变性研究。在亚慢性毒性研究中，Sprague Dawley 大鼠（每组 12 只/性别）经口给予（灌胃）蘑菇 β-葡聚糖，剂量水平分别为 0、500、1000 和 2000 mg/kg 体重/天，共 90 天。与对照组相比，β-葡聚糖给药未导致临床观察、眼科检查、体重、体重增加、饲料消耗和器官重量出现任何毒理学上有意义的与治疗相关的变化。β-葡聚糖对血液学、血清化学参数、尿液分析或终端剖检（大体或组织病理学发现）没有任何不良影响。通过鼠伤寒沙门氏菌的基因突变、体外染色体畸变和体内微核试验评估的致突变性研究结果表明，β-葡聚糖没有遗传毒性。基于亚慢性研究，确定蘑菇 β-葡聚糖的无观察到不良效应水平（NOAEL）为 2000 mg/kgbw/天，这是测试的最高剂量。
