免疫调节剂“蘑菇β葡聚糖”诱导 Wnt/β 连环蛋白信号通路并改善罗非鱼和大鼠皮肤伤口愈合：一项组织病理学研究。
Immunomodulator 'mushroom beta glucan' induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study.
机构信息
Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan.
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
出版信息
Int Wound J. 2016 Dec;13(6):1116-1128. doi: 10.1111/iwj.12427. Epub 2015 Apr 10.
Abstract
The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β-catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery.
摘要
本研究旨在探讨蘑菇β葡聚糖 (MBG) 对尼罗罗非鱼部分肝切除 (PH) 和大鼠皮肤伤口愈合检查中伤口恢复的影响。在 PH 后，我们专注于使用体外和体内试验研究其对肝脏修复能力的影响。在体外，我们通过 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐 (MTT) 测定法和溴脱氧尿苷 (BrdU) 细胞增殖测定法检测方法，检测 MBG 是否对肝实质细胞的增殖有影响。结果表明，MBG 处理可显著增强肝实质细胞的增殖能力，维持细胞活力。免疫组织化学染色分析 Wnt/β-catenin 信号通路也表明，MBG 具有促进 PH 术后肝组织细胞增殖的作用。
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