具有内源性催化活性的稳定酶-辅酶复合物的制备。
The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.
作者信息
Gacesa P, Venn R F
出版信息
Biochem J. 1979 Jan 1;177(1):369-72. doi: 10.1042/bj1770369.
Abstract
N6-(6-Aminohexylcarbamoylmethyl)-NAD+ was coupled to lactate dehydrogenase by using glutaraldehyde to form an active complex. The stability of this complex could be considerably improved by reduction with KBH4, although this treatment caused a partial decrease in specific activity. NAD+ was also coupled directly to the enzyme by this method. All of these complexes exhibited an intrinsic activity in the absence of exogenous NAD+.
摘要
通过使用戊二醛将N6-(6-氨基己基氨甲酰甲基)-NAD⁺与乳酸脱氢酶偶联，形成活性复合物。用KBH₄还原可显著提高该复合物的稳定性，尽管这种处理会导致比活性部分降低。NAD⁺也可通过此方法直接与该酶偶联。所有这些复合物在没有外源NAD⁺的情况下均表现出内在活性。
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The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.通过戊二醛偶联和硼氢化钠还原将腺嘌呤核苷酸固定在多糖上。
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