Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
J Bacteriol. 2011 Oct;193(20):5824-32. doi: 10.1128/JB.05456-11. Epub 2011 Aug 19.
Salmonella enterica serovar Typhimurium can utilize molecular hydrogen for growth and amino acid transport during anaerobic growth. Via microarray we identified H(2) gas-affected gene expression changes in Salmonella. The addition of H(2) caused altered expression of 597 genes, of which 176 genes were upregulated and 421 were downregulated. The significantly H(2)-upregulated genes include those that encode proteins involved in the transport of iron, manganese, amino acids, nucleosides, and sugars. Genes encoding isocitrate lyase (aceA) and malate synthase (aceB), both involved in the carbon conserving glyoxylate pathway, and genes encoding the enzymes of the d-glucarate and d-glycerate pathways (gudT, gudD, garR, garL, garK) are significantly upregulated by H(2). Cells grown with H(2) showed markedly increased AceA enzyme activity compared to cells without H(2). Mutant strains with deletion of either aceA or aceB had reduced H(2)-dependent growth rates. Genes encoding the glutamine-specific transporters (glnH, glnP, glnQ) were upregulated by H(2), and cells grown with H(2) showed increased [(14)C]glutamine uptake. Similarly, the mannose uptake system genes (manX, manY) were upregulated by H(2,) and cells grown with H(2) showed about 2.0-fold-increased [(14)C]d-mannose uptake compared to the cells grown without H(2). Hydrogen stimulates the expression of genes involved in nutrient and carbon acquisition and carbon-conserving pathways, linking carbon and energy metabolism to sustain H(2)-dependent growth.
鼠伤寒沙门氏菌血清型 Typhimurium 可以在厌氧生长过程中利用分子氢进行生长和氨基酸运输。通过微阵列，我们确定了 H2 气体对沙门氏菌基因表达变化的影响。添加 H2 导致 597 个基因的表达发生改变，其中 176 个基因上调，421 个基因下调。受 H2 显著上调的基因包括编码参与铁、锰、氨基酸、核苷和糖运输的蛋白质的基因。编码异柠檬酸裂解酶（aceA）和苹果酸合酶（aceB）的基因，都参与碳保存的乙醛酸途径，以及编码 d-葡萄糖酸盐和 d-甘油酸盐途径的酶的基因（gudT、gudD、garR、garL、garK），都被 H2 显著上调。与没有 H2 的细胞相比，用 H2 培养的细胞显示出明显增加的 AceA 酶活性。缺失 aceA 或 aceB 的突变株的 H2 依赖性生长速率降低。编码谷氨酰胺特异性转运蛋白（glnH、glnP、glnQ）的基因被 H2 上调，用 H2 培养的细胞显示出增加的 [(14)C]谷氨酰胺摄取。同样，甘露糖摄取系统基因（manX、manY）也被 H2 上调，与没有 H2 生长的细胞相比，用 H2 生长的细胞显示出大约 2.0 倍的 [(14)C]d-甘露糖摄取增加。氢气刺激参与营养物质和碳获取以及碳保存途径的基因的表达，将碳和能量代谢联系起来，以维持 H2 依赖性生长。
