Celis J E, Crüger D, Kiil J, Lauridsen J B, Ratz G, Basse B, Celis A
Institute of Medical Biochemistry, Aarhus University, Denmark.
FEBS Lett. 1990 Mar 26;262(2):159-64. doi: 10.1016/0014-5793(90)80179-m.
Analysis using two-dimensional (2D) gel electrophoresis of the [35S]-methionine-labelled proteins synthesized by non-cultured total epidermal keratinocytes obtained from normal and psoriatic skin revealed 6 proteins that are strongly up-regulated (5 times or more) in psoriatic skin. These proteins are synthesized at albeit lower levels by keratinocytes from normal and normal-appearing (uninvolved) skin of psoriatic patients, and correspond to isoelectric focusing sample spot numbers 4311 (40.3 kDa), 4003 (12.4 kDa), 5008 (11.9 kDa), 3012 (11.6 kDa), 6016 (11.6 kDa) and 1015 (10.1 kDa) in the normal keratinocyte 2D gel protein database [Celis et al, (1990) Electrophoresis, in press]. These proteins are also detected in the labelling medium indicating that they are at least in part secreted. Given their striking regulatory behavior, these proteins may play a role in the pathogenesis of psoriasis.
通过二维（2D）凝胶电泳分析从正常皮肤和银屑病皮肤获取的未培养的全层表皮角质形成细胞合成的[35S] - 甲硫氨酸标记蛋白，发现有6种蛋白在银屑病皮肤中强烈上调（5倍或更多）。这些蛋白在银屑病患者的正常皮肤和外观正常（未受累）皮肤的角质形成细胞中也有合成，只是水平较低，并且对应于正常角质形成细胞二维凝胶蛋白数据库[Celis等人，（1990年）《电泳》，即将发表]中的等电聚焦样品斑点编号4311（40.3 kDa）、4003（12.4 kDa）、5008（11.9 kDa）、3012（11.6 kDa）、6016（11.6 kDa）和1015（10.1 kDa）。在标记培养基中也检测到了这些蛋白，表明它们至少部分是分泌型的。鉴于它们显著的调节行为，这些蛋白可能在银屑病的发病机制中起作用。
