Département de Biologie et Ecologie végétales, Université d'Antananarivo, BP 906 Antananarivo, Madagascar.
Am J Bot. 2012 Apr;99(4):e176-8. doi: 10.3732/ajb.1100441. Epub 2012 Apr 2.
Microsatellite markers for Centella asiatica, an important medicinal herb, were developed and characterized to promote genetic and molecular studies.
A GA/GT-enriched genomic library was constructed from an accession from Madagascar. Roughly 75% of the 768 clones of the enriched library contained microsatellites. Eighty sequences containing microsatellites were obtained from 96 positive clones. Specific primers were designed for 20 loci, and 17 of them displayed polymorphism when screened across 17 C. asiatica accessions, with an average of 4.3 alleles per locus. The observed and expected heterozygosity values averaged 0.114 and 0.379, respectively.
This is the first report constructing an enriched genomic library and identifying microsatellite markers from C. asiatica. These 17 polymorphic microsatellite markers are a useful resource for this plant, applicable for diversity studies, pedigree analyses, and genetic mapping.
开发和鉴定了作为重要药用植物的积雪草的微卫星标记，以促进其遗传和分子研究。
从马达加斯加的一个样本中构建了一个 GA/GT 富集基因组文库。大约 75%的 768 个富集文库克隆包含微卫星。从 96 个阳性克隆中获得了 80 个含有微卫星的序列。针对 20 个位点设计了特异性引物，在对 17 个积雪草样本进行筛选时，其中 17 个显示出多态性，每个位点的平均等位基因数为 4.3 个。观察到的和预期的杂合度平均值分别为 0.114 和 0.379。
这是首次从积雪草中构建富集基因组文库并鉴定微卫星标记的报告。这 17 个多态性微卫星标记是该植物的有用资源，可用于多样性研究、系谱分析和遗传作图。
