John S, Balagurunathan R
Department of Biochemistry, Indian Institute of science, Bangalore - 560 012, India.
Indian J Med Microbiol. 2011 Jul-Sep;29(3):302-4. doi: 10.4103/0255-0857.83918.
This study aims in identifying MBLs particularly Zn requiring Molecular Class B enzymes produced by Pseudomonas aeruginosa and Acinetobacter baumannii .The resistance by these organisms are in a rise against all antibiotics including carbapenems and no prescribed CLSI guidelines is available for detecting them. Clinical isolates antibiotic susceptibility was determined by number of phenotypic tests by addition of 50mM of 10 μl zinc as cofactor for metallo beta lactamase production along with 0.5M ETDA of 5 μl (930 μg per disk) plain disks. Increase in zone size of the meropenem -EDTA disk compared to the meropenem disk without EDTA was recorded positive. For Zn requiring MBLs zone towards both disks of EDTA and Zn along with meropenem is detected by DDST.
本研究旨在鉴定由铜绿假单胞菌和鲍曼不动杆菌产生的金属β-内酰胺酶（MBLs），特别是需要锌的B类分子酶。这些微生物对包括碳青霉烯类在内的所有抗生素的耐药性正在上升，并且没有可用的规定CLSI指南来检测它们。通过添加50mM的10μl锌作为金属β-内酰胺酶产生的辅因子以及5μl（每片930μg）的0.5M乙二胺四乙酸（EDTA）普通纸片，通过多种表型试验确定临床分离株的抗生素敏感性。与没有EDTA的美罗培南纸片相比，美罗培南-EDTA纸片的抑菌圈增大记录为阳性。对于需要锌的MBLs，通过双碟协同试验（DDST）检测EDTA、锌与美罗培南两种纸片的抑菌圈。
