Alves Stênio Nunes, Tibúrcio Jacqueline Domingues, Melo Alan Lane de
Universidade Federal de São João del-Rei, Divinópolis, MG.
Rev Soc Bras Med Trop. 2011 Jul-Aug;44(4):486-9. doi: 10.1590/s0037-86822011000400017.
This study aimed to assess the susceptibility of Culex quinquefasciatus larvae to two pyrethroids (Cypermethrin and Deltamethrin), two derivatives of Avermectin (Ivermectin and Abamectin) and an organophosphate (Temephos).
Third- and fourth-instar larvae of C. quinquefasciatus were exposed to different concentrations of insecticides (eleven repetitions) according to the World Health Organization's protocol. One hour after exposure, larvae were washed in dechlorinated tap water, transferred to plastic containers containing water without chlorine, fed and observed for periods of 24h until reaching adulthood. To determine lethal concentrations, the obtained values were submitted to regression analysis using the probit model with the Minitab 15 program.
The highest concentration used for the LC50 from probit analysis was for Temephos. The evaluated insecticides caused more pronounced larvae mortality in the first 24h with the exception of those exposed to ivermectin.
This study demonstrates that larvae are susceptible to all tested insecticides and that there is a need for monitoring the use of insecticides.
本研究旨在评估致倦库蚊幼虫对两种拟除虫菊酯(氯氰菊酯和溴氰菊酯)、两种阿维菌素衍生物(伊维菌素和阿维菌素)以及一种有机磷杀虫剂(双硫磷)的敏感性。
根据世界卫生组织的方案,将致倦库蚊的三龄和四龄幼虫暴露于不同浓度的杀虫剂中(十一次重复)。暴露一小时后,将幼虫在去氯自来水中冲洗,转移至装有无氯水的塑料容器中,喂食并观察24小时直至成虫。为确定致死浓度,使用Minitab 15程序通过概率模型对所得值进行回归分析。
概率分析中用于计算半数致死浓度(LC50)的最高浓度是双硫磷的。除暴露于伊维菌素的幼虫外,所评估的杀虫剂在最初24小时内导致更明显的幼虫死亡。
本研究表明幼虫对所有测试杀虫剂敏感,并且有必要监测杀虫剂的使用情况。