BioTherapeutics Research Laboratory, Robarts Research Institute, University of Western Ontario, London, Ontario, Canada.
Contrast Media Mol Imaging. 2011 Jul-Aug;6(4):314-27. doi: 10.1002/cmmi.433.
An optimized non-invasive imaging modality capable of tracking and quantifying in vivo DC migration in patients would provide clinicians with valuable information regarding therapeutic DC-based vaccine outcomes. Superparamagnetic iron oxide (SPIO) nanoparticles were used to label bone marrow-derived DC. In vivo DC migration was tracked and quantified non-invasively using cellular magnetic resonance imaging (MRI) in a mouse model. Labelling DC with SPIO reflects the kinetics of DC migration in vivo but appears to reduce overall DC migration, in part due to nanoparticle size. Magnetic separation of SPIO-labelled (SPIO(+)) DC from unlabelled (SPIO(-)) DC prior to injection improves SPIO(+) DC migration to the lymph node. Corresponding MR image data better correlate with the presence of DC in vivo; an improved immunological response is also seen. Cellular MRI is a viable, non-invasive imaging tool that can routinely track DC migration in vivo. Consideration should be given to optimizing MRI contrast agent-labelling of clinical-grade DC in order to accurately correlate DC fate to immunological outcomes in patients.
一种优化的非侵入性成像方式，能够对体内的 DC 迁移进行跟踪和定量分析，这将为临床医生提供有关基于治疗性 DC 的疫苗效果的有价值信息。超顺磁性氧化铁 (SPIO) 纳米颗粒被用于标记骨髓来源的 DC。在小鼠模型中，使用细胞磁共振成像 (MRI) 对体内的 DC 迁移进行非侵入性跟踪和定量分析。SPIO 标记的 DC 标记 (SPIO(+)) 反映了体内 DC 迁移的动力学，但似乎会减少整体 DC 迁移，部分原因是纳米颗粒的大小。在注射前将 SPIO 标记的 (SPIO(+)) DC 与未标记的 (SPIO(-)) DC 进行磁性分离，可提高 SPIO(+)DC 向淋巴结的迁移。相应的 MR 图像数据与体内 DC 的存在相关性更好；还观察到免疫反应得到改善。细胞 MRI 是一种可行的、非侵入性的成像工具，可常规跟踪体内的 DC 迁移。应该考虑优化 MRI 造影剂标记临床级别的 DC，以便准确地将 DC 的命运与患者的免疫结果相关联。
